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Simple and Sensitive Paper-based Colorimetric Biosensor for Determining Total Polyphenol Content of the Green Tea Beverages

  • Research Paper
  • Nanobiotechnology
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Abstract

A simple paper-based colorimetric biosensor based on immobilized tyrosinase and 3-methyl-2-benzothiazolinone hydrazone (MBTH) was developed for assessing total polyphenol content (TPC) of various green tea beverages. The patterned biosensing zone of the paperbased biosensor showed a sensitive response to catechin (a typical green tea polyphenol) by generating pink color adducts that can be captured for further color image analysis using a scanometric method. This color change can be connected to the TPC of the samples. The analytical performance of the biosensor in scanometric set-up was optimized. The biosensor showed a response time of 13 min in the linear range between 0.08-1.03 mM of catechin (r = 0.9979). The detection limit (LOD) of the biosensor was 0.071 mM while reproducibility was found at 3.11% RSD (relative standard deviation). TPC of green tea beverages was assessed by the biosensor, and the results were in accordance with the UV/Vis spectrophotometric method.

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Acknowledgments

The authors would like to thank Chemo & Biosensor Group at Faculty of Pharmacy, The University of Jember for supporting this work. The authors declare that they have no conflict of interest. Neither ethical approval nor informed consent was required for this study.

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Authors and Affiliations

  1. Pharmaceutical Chemistry Department, Faculty of Pharmacy, Airlangga University, Dharmawangsa Dalam 4-6 Surabaya, East Java, 60286, Indonesia

    Mochammad Amrun Hidayat, Djoko Agus Purwanto & Mochammad Yuwono

  2. Chemo & Biosensor Group, Faculty of Pharmacy, University of Jember, Kalimantan I/2 Tegal Boto Campus Jember, East Java, 68121, Indonesia

    Mochammad Amrun Hidayat, Diah Ayu Maharani & Bambang Kuswandi

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  1. Mochammad Amrun Hidayat
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  2. Diah Ayu Maharani
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Correspondence to Mochammad Amrun Hidayat.

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Hidayat, M.A., Maharani, D.A., Purwanto, D.A. et al. Simple and Sensitive Paper-based Colorimetric Biosensor for Determining Total Polyphenol Content of the Green Tea Beverages. Biotechnol Bioproc E 25, 255–263 (2020). https://doi.org/10.1007/s12257-019-0299-8

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  • DOI: https://doi.org/10.1007/s12257-019-0299-8

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