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Effects of Time on Phenolics and in vitro Bioactivity in Autoclave Extraction of Graviola (Annona muricata) Leaf

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  • Biomedical Engineering
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Abstract

We investigated the effects of different autoclave extraction times (1-6 h) on the antioxidant, antidiabetic, and anti-inflammatory activities of phenolic compounds isolated from graviola (Annona muricata) leaves. In terms of yield and content of total polyphenols, total flavonoids, rutin, and kaempferol-3-O-rutinoside, the optimal extraction time was 4 h. At this time point, water extracts from graviola leaves (WEG) exhibited the highest DPPH radical scavenging activity, ferric reducing antioxidant power, and α-glucosidase inhibitory activity. The α-glucosidase inhibitory activity of WEG was significantly stronger than that of acarbose. Furthermore, WEG significantly suppressed lipopolysaccharide-induced nitric oxide production in RAW 264.7 cells and inhibited UVB-induced activator protein-1 (AP-1)-dependent transcription in human keratinocytes. In conclusion, WEG can be used as functional food ingredients in the food industry.

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Acknowledgements

This work was supported by the Technology development Program (C0452780) funded by the Ministry of SMEs and Startups (MSS, Korea).

The authors declare no conflict of interest.

Neither ethical approval nor informed consent was required for this study.

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Authors and Affiliations

  1. Department of Food and Nutrition, and Bionanocomposite Research Center, Kyung Hee University, Seoul, 02447, Korea

    Moonkyeung Choi, Yu-Ra Kang & Yoon Hyuk Chang

  2. Department of Food Bioscience and Technology, Korea University, Seoul, 02841, Korea

    Hyo Don Zu

  3. Chamhan Food, Inc., Gyeonggi-do, 11018, Korea

    In-Sook Lim

  4. School of Food Science and Biotechnology, Kyungpook National University, Daegu, 41556, Korea

    Sung Keun Jung

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  1. Moonkyeung Choi
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Choi, M., Kang, YR., Zu, H.D. et al. Effects of Time on Phenolics and in vitro Bioactivity in Autoclave Extraction of Graviola (Annona muricata) Leaf. Biotechnol Bioproc E 25, 9–15 (2020). https://doi.org/10.1007/s12257-019-0259-3

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  • DOI: https://doi.org/10.1007/s12257-019-0259-3

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