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Ectopic Overexpression of Teff Grass (Eragrostis tef) Phi-class Glutathione S-transferase 1 (EtGSTF1) Enhances Prokaryotic Cell Survivability against Diverse Abiotic Stresses

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Abstract

The glutathione S-transferases (GSTs) are encoded by a large gene family and well conserved in all living organisms; however they have evolved and are classified clearly according to each kingdom. GSTs are a dimeric protein that has been reported to maintain redox homeostasis in cells, and to protect organisms against oxidative damage. Recently, we isolated a GST coding gene from stress-treated teff grass (Eragrostis tef) and identified it as a plant-specific phi class GST (EtGSTF1) possessing conserved phi class-specific GST N- and C-terminal domains, GSH binding site, substrate binding pocket, and dimer interface. We found that overexpression of plant-specific phi class EtGSTF1 confers diverse abiotic stress tolerances including salt, osmotic, and heat stresses in E. coli which does not possess phi class GSTs. In addition, EtGSTF1 expression helps the E. coli cells tolerate arsenic (As)-induced cell toxicity. Collectively, although plantae and prokaryotae have differentiated a few billions of years ago, the plant-specific phi class EtGSTF1 could protect prokaryotic organisms by detoxification of molecules under diverse abiotic stresses.

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Acknowledgments

This research work was supported by the “Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ012187)”. This study was also supported by Postdoctoral Fellowship Program of National Institute of Animal Science funded by Rural Development Administration (RDA), Republic of Korea.

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  1. These authors contributed equally to the work.

Authors and Affiliations

  1. Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration, Cheonan, 31000, Korea

    Ki-Won Lee, Md. Atikur Rahman & Hee Chung Ji

  2. Department of Plant Medicine, Gyeongsang National University, Jinju, 52828, Korea

    Serim Hong & Daeyoung Son

  3. Division of Applied Life Science (BK21 Plus), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju, 52828, Korea

    Joon-Yung Cha & Daeyoung Son

  4. Feed and Forage Biosciences, International Livestock Research Institute, Addis Ababa, 5689, Ethiopia

    Chris Stephen Jones

  5. Animal Genetic Resources Research Center, National Institute of Animal Science, Rural Development Administration, Namwon, 55717, Korea

    Sang-Hoon Lee

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  1. Ki-Won Lee
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Correspondence to Daeyoung Son or Sang-Hoon Lee.

Electronic supplementary material

12257_2018_495_MOESM1_ESM.pdf

Supplementary Fig. 1. Sequence of teff grass GSTF1 (EtGSTF1). Nucleotide and deduced amino acid sequence of EtGSTF1. Numbers at left indicate the nucleotide (up) and amino acid residue (down) position.

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Lee, KW., Hong, S., Rahman, M.A. et al. Ectopic Overexpression of Teff Grass (Eragrostis tef) Phi-class Glutathione S-transferase 1 (EtGSTF1) Enhances Prokaryotic Cell Survivability against Diverse Abiotic Stresses. Biotechnol Bioproc E 24, 552–559 (2019). https://doi.org/10.1007/s12257-018-0495-y

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  • DOI: https://doi.org/10.1007/s12257-018-0495-y

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