Biotechnology and Bioprocess Engineering

, Volume 24, Issue 1, pp 240–249 | Cite as

Fluorescence-based Quantification of Bioactive Keratin Peptides from Feathers for Optimizing Large-scale Anaerobic Fermentation and Purification

  • Hyeon-Su Jin
  • Seon Yeong Park
  • Ji-Yeon Kim
  • Jae-Eun Lee
  • Han-Seung Lee
  • Nam Joo Kang
  • Dong-Woo LeeEmail author
Research Paper


The extremely thermophilic eubacterium Fervidobacterium islandicum AW-1 produces low molecular weight (LMW; < 1 kDa) keratin peptides (KPs) from poultry feathers at 70°C. However, detection and quantification of feather hydrolysate-derived peptides is needed for optimizing fermentation and down-stream processes. Herein, we developed a large-scale fermentation and purification of skin anti-aging LMW KPs from recalcitrant feathers using fluorescence-based quantification of N-terminal prolinecontaining KPs derivatized with 3,4-dihydroxybenzoic acid to yield fluorescent adducts. Fluorescent products were correlated with bioactive KP concentrations in keratin fractions and cosmetic formulations. Subsequent anaerobic fermentative keratinolysis and large-scale purification achieved 4.4 g/L LMW KPs from 8 g/L native feathers in a 5 L batch bioreactor, generating 0.8 g/L purified MMP-1 suppressive KPs (yield = 1.2%). This demonstrated the feasibility of industrial-scale anaerobic feather digestion and purification of LMW KPs to produce skin anti-aging peptides from keratin hydrolysates in a more environmentally sustainable manner.


Fervidobacterium islandicum LMW keratin peptide anaerobic digestion large-scale purification skin anti-aging 


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Copyright information

© The Korean Society for Biotechnology and Bioengineering and Springer 2019

Authors and Affiliations

  • Hyeon-Su Jin
    • 1
  • Seon Yeong Park
    • 2
  • Ji-Yeon Kim
    • 2
  • Jae-Eun Lee
    • 3
  • Han-Seung Lee
    • 4
  • Nam Joo Kang
    • 3
  • Dong-Woo Lee
    • 1
    Email author
  1. 1.Department of BiotechnologyYonsei UniversitySeoulKorea
  2. 2.School of Applied BiosciencesKyungpook National UniversityDaeguKorea
  3. 3.School of Food Science and BiotechnologyKyungpook National UniversityDaeguKorea
  4. 4.Major in Food BiotechnologySilla UniversityBusanKorea

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