Skip to main content
SpringerLink
Log in

Function and Application Analysis of Ac132 Protein in Autographa californica Multiple Nucleopolyhedrovirus

  • Research Paper
  • Published:
Biotechnology and Bioprocess Engineering Aims and scope Submit manuscript

Abstract

Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is used as bac-to-bac eukaryotic protein expression system and biopesticide. However, AcMNPV isn’t widely used in protein expression system and biological control due to some drawbacks and it’s required to understand the function of AcMNPV genes comprehensively in the infection process. Ac132 is an improtant component of the budded virus (BV) nucleocapsid of AcMNPV and the function of Ac132 in regulating progeny virus generation is still unclear. In this study, the recombinant AcMNPV-Ac132-EGFP was constructed. Virus propagation assay indicated that overexpression of Ac132 could improve progeny virus production. Moreover, fluorescent microscope and western blot analysis showed AcMNPV-Ac132-EGFP could accelerate the aggregation of F-actin in the nucleus and delay the evacuation process. In addition, AcMNPV-Ac132-EGFP boosted the utilization of glucose in Sf9 cells at the aspect of energy metabolism. These results demonstrated that Ac132 played important roles in progeny virus production, F-actin formation, nucleocapsid transportion, and glucose metabolism in Sf9 cells, which provided an improved bac-to-bac eukaryotic protein expression system and biopesticide.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Ayres, M. D., S. C. Howard, J. Kuzio, M. Lopez–Ferber, and R. D. Possee (1994) The complete DNA sequence of Autographa californica nuclear polyhedrosis virus. Virology 202: 586–605.

    Article  CAS  PubMed  Google Scholar 

  2. Wang, R., F. Deng, D. Hou, Y. Zhao, L. Guo, H. Wang, and Z. Hu (2010) Proteomics of the Autographa californica nucleopolyhedrovirus buddedvirions. J. Virol. 84: 7233–7242.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Braunagel, S. C., W. K. Russell, G. Rosas–Acosta, D. H. Russell, and M. D. Summers (2003) Determination of the protein composition of the occlusion–derived virus of Autographa californica nucleopolyhedrovirus. Proc. Natl. Acad. Sci. USA 100: 9797–9802.

    Article  CAS  PubMed  Google Scholar 

  4. Chen, Y. R., S. Zhong, Z. Fei, Y. Hashimoto, J. Z. Xiang, S. Zhang, and G. W. Blissard (2013) The transcriptome of the baculovirus Autographa californicamultiple nucleopolyhedrovirus in Trichoplusia ni cells. J. Virol. 87:6391–6405.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Rohrmann, G. F. and M. D. Bethesda (2013) Baculovirus molecular biology, 3rd ed. National Center for Biotechnology Information, National Library of Medicine.

    Google Scholar 

  6. Miele, S. A., M. J. Garavaglia, M. N. Belaich, and P. D. Ghiringhelli (2011) Baculovirus: molecular insights on their diversity and conservation. Int. J. Evol. Biol. 2011:379424.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Yang, M., S. Wang, X. L. Yue, and L. L. Li (2014) Autographa californica multiple nucleopolyhedrovirus orf132 encodes a nucleocapsid–associated protein required for budded–virus and multiply enveloped occlusion–derived virus production. J. Virol. 88: 12586–12598.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Fang, Z., C. Li, W. Wu, M. Yuan, and K. Yang (2016) The Autographa californica multiple nucleopolyhedro virus Ac132 plays a role in nuclear entry. J. Gen. Virol. 97:3030–3038.

    Article  CAS  PubMed  Google Scholar 

  9. Young, J. C., E. A. MacKinnon, and P. Faulkner (1993) The architecture of the virogenic stroma in isolated nuclei of Spodoptera frugiperda cells in vitro infected by Autographa californica nuclear polyhedrosis virus. J. Struct. Biol. 110: 141–153.

    Article  Google Scholar 

  10. Fu, Y. J., T. T. Lin, A. H. Liang, and F. Hu (2016) Effects of recombinant baculovirus AcMNPV–BmK IT on the formation of early cables and nuclear polymerization of actin in Sf9 cells. Cytotechnology 68: 381–387.

    Article  CAS  PubMed  Google Scholar 

  11. Au, S., W. Wu, L. Zhou, D. A. Theilmann, and N. Panté (2016) A new mechanism for nuclear import by actin–based propulsion used by a baculovirus nucleocapsid. J. Cell Sci. 129: 2905–2911.

    Article  CAS  PubMed  Google Scholar 

  12. Hepp, S. E., G. M. Borgo, S. Ticau, T. Ohkawa, and M. D. Welch (2018) Baculovirus AC102 is a nucleocapsid protein that is crucial for nuclear actin polymerization and nucleocapsid morphogenesis. J. Virol. 92: 11–17.

    Article  Google Scholar 

  13. Zhang, Y., X. Hu, J. Mu, Y. Hu, Y. Zhou, H. Zhao, C. Wu, R. Pei, J. Chen, X. Chen, and Y. Wang (2018) Ac102 participates in nuclear actin polymerization by modulating BV/ODV–C42 Ubiquitination during Autographa californica multiple nucleopolyhedrovirus infection. J Virol. 92:12–14.

    Google Scholar 

  14. Xiao, W., Y. Yang, Q. Weng, T. Lin, M. Yuan, K. Yang, and Y. Pang (2009) The role of the PI3K–Akt signal transduction pathway in Autographa californica multiple nucleopolyhedrovirus infection of Spodoptera frugiperda cells. Virology 391: 83–89.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan, 030006, China

    Yueyou Peng, Aihua Liang & Yuejun Fu

  2. College of Life Science, Shanxi University, Taiyuan, 030006, China

    Yuejun Fu

Authors
  1. Yueyou Peng
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Aihua Liang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yuejun Fu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yuejun Fu.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Peng, Y., Liang, A. & Fu, Y. Function and Application Analysis of Ac132 Protein in Autographa californica Multiple Nucleopolyhedrovirus. Biotechnol Bioproc E 23, 655–661 (2018). https://doi.org/10.1007/s12257-018-0364-8

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12257-018-0364-8

Keywords

Search

Navigation