Biotechnology and Bioprocess Engineering

, Volume 23, Issue 5, pp 573–579 | Cite as

Enhancement of Pyruvate Productivity in Candida glabrata by Deleting the CgADE13 Gene to Improve Acid Tolerance

  • Xiulai ChenEmail author
  • Qiuling Luo
  • Jia Liu
  • Liming Liu
Research Paper


Acid tolerance is one of the critical factors to evaluate the quality of the industrial production strains, especially organic acid producing microorganisms. To circumvent this problem, we investigated the physiological function of adenylosuccinate lyase in AMP metabolism from Candida glabrata by deleting the corresponding gene, CgADE13. At pH 4.0, CgADE13 deletion resulted in a 68.3% and 112.0% increase in biomass and cell viability compared to those of wild type strain (wt), respectively. In addition, CgADE13 deletion also protected cell morphology and counteracted ROS production. Further, the intracellular ATP level of strain Cgade13Δ was decreased by 25.0%, and its H+-ATPase activity was increased by 15.0%. Finally, pyruvate production with strain Cgade13Δ in a 30-L batch bioreactor at pH 4.0 reached 53.9 g/L, and pyruvate productivity was increased by 166.7% compared to that of wt. This is the first report regarding tolerance engineering of C. glabrata for enhancing pyruvate productivity, which provides a good starting point for metabolic engineering to achieve the industrial production of other chemicals.


adenylosuccinate lyase ATP acid tolerance pyruvate Candida glabrata 


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Copyright information

© The Korean Society for Biotechnology and Bioengineering and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Xiulai Chen
    • 1
    • 2
    Email author
  • Qiuling Luo
    • 1
    • 2
  • Jia Liu
    • 1
    • 2
  • Liming Liu
    • 1
    • 2
    • 3
  1. 1.State Key Laboratory of Food Science and TechnologyJiangnan UniversityWuxiChina
  2. 2.Key Laboratory of Industrial Biotechnology, Ministry of EducationJiangnan UniversityWuxiChina
  3. 3.National Engineering Laboratory for Cereal Fermentation TechnologyJiangnan UniversityWuxiChina

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