Abstract
Lipid extraction is a critical step in the downstream processing of biodiesel production from microalgae. Solvent extraction using mixtures of non-polar and polar solvents is one of the most well-known processes for this purpose. Hexane is the most common solvent of choice for large-scale lipid extractions due to its technical and economic advantages, especially its high selectivity toward lipids and low cost. In this study, extractions using mixtures of hexane and polar solvents were evaluated for their performance in order to develop a more efficient method for large-scale lipid extraction from microalgae. The combination of hexane and methanol resulted in the highest fatty acid methyl ester (FAME) yield for lipids from Tetraselmis sp. The effects of extraction conditions, including proportions of methanol to hexane, ratios of total solvent volume to dry biomass, and extraction time, on extraction yields were evaluated to determine optimum conditions providing higher lipid and FAME yields. The optimal conditions were as follows: proportion of hexane to methanol of 1:1, ratio of total solvent volume to dry biomass of 10 mL/g, and extraction time of 120 min. Finally, the selected solvent mixture and optimal conditions were applied to larger scale extraction experiments with scale-up factors of 10, 50, and 100. FAME yields of large-scale extractions were almost completely consistent with increasing scale-up factors. The results of this study suggest that a hexane and methanol mixture is a promising solvent for large-scale lipid extraction from microalgae.
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Chai, F., F. Cao, F. Zhai, Y. Chen, X. Wang, and Z. Su (2007) Transesterification of vegetable oil to biodiesel using a heteropolyacid solid catalyst. Adv. Synth. Catal. 349: 1057–1065.
Muthu, H., V. SathyaSelvabala, T. Varathachary, D. Kirupha Selvaraj, J. Nandagopal, and S. Subramanian (2010) Synthesis of biodiesel from Neem oil using sulfated zirconia via tranesterification. Braz. J. Chem. Eng. 27: 601–608.
Sahoo, P. and L. Das (2009) Combustion analysis of Jatropha, Karanja and Polanga based biodiesel as fuel in a diesel engine. Fuel 88: 994–999.
Balat, M. and H. Balat (2010) Progress in biodiesel processing. Appl. Energy. 87: 1815–1835.
Liu, Z.-X., X.-A. Nie, and Y.-G. Wang (2013) Study on technology of comprehensive utilization of biodiesel and dimers from rapeseed oil. J. Forest Prod. Indust. 2: 5–8.
Sheehan, J., V. Camobreco, J. Duffield, M. Graboski, and H. Shapouri (1998) Life cycle inventory of biodiesel and petroleum diesel for use in an urban bus. National Renewable Energy Laboratory, Golden, CO, USA.
Daroch, M., S. Geng, and G. Wang (2013) Recent advances in liquid biofuel production from algal feedstocks. Appl. Energy 102: 1371–1381.
Chisti, Y. (2007) Biodiesel from microalgae. Biotechnol. Adv. 25: 294–306.
Rosenberg, J. N., G. A. Oyler, L. Wilkinson, and M. J. Betenbaugh (2008) A green light for engineered algae: Redirecting metabolism to fuel a biotechnology revolution. Curr. Opin. Biotechnol. 19: 430–436.
Sheehan, J., T. Dunahay, J. Benemann, and P. Roessler (1998) A look back at the US Department of Energy’s aquatic species program: biodiesel from algae. National Renewable Energy Laboratory, Golden, CO, USA.
Schenk, P. M., S. R. Thomas-Hall, E. Stephens, U. C. Marx, J. H. Mussgnug, C. Posten, O. Kruse, and B. Hankamer (2008) Second generation biofuels: High-efficiency microalgae for biodiesel production. Bioenergy Res. 1: 20–43.
Widjaja, A., C.-C. Chien, and Y.-H. Ju (2009) Study of increasing lipid production from fresh water microalgae Chlorella vulgaris. J. Taiwan Inst. Chem. Eng. 40: 13–20.
Halim, R., M. K. Danquah, and P. A. Webley (2012) Extraction of oil from microalgae for biodiesel production: A review. Biotechnol. Adv. 30: 709–732.
Neto, A. M. P., R. A. S. de Souza, A. D. Leon-Nino, J. D. A. A. da Costa, R. S. Tiburcio, T. A. Nunes, T. C. S. de Mello, F. T. Kanemoto, F. M. P. Saldanha-Corrêa, and S. M. F. Gianesella (2013) Improvement in microalgae lipid extraction using a sonication-assisted method. Renew. Energy 55: 525–531.
Lee, S. J., B.-D. Yoon, and H.-M. Oh (1998) Rapid method for the determination of lipid from the green alga Botryococcus braunii. Biotechnol. Tech. 12: 553–556.
Medina, A. R., E. M. Grima, A. G. Giménez, and M. I. González (1998) Downstream processing of algal polyunsaturated fatty acids. Biotechnol. Adv. 16: 517–580.
Halim, R., B. Gladman, M. K. Danquah, and P. A. Webley (2011) Oil extraction from microalgae for biodiesel production. Bioresour. Technol. 102: 178–185.
Cheng, C.-H., T.-B. Du, H.-C. Pi, S.-M. Jang, Y. H. Lin, and H.-T. Lee (2011) Comparative study of lipid extraction from microalgae by organic solvent and supercritical CO2. Bioresour. Technol. 102: 10151–10153.
Shin, H.-Y., J.-H. Ryu, S.-Y. Bae, C. Crofcheck, and M. Crocker (2014) Lipid extraction from Scenedesmus sp. microalgae for biodiesel production using hot compressed hexane. Fuel 130: 66–69.
Malekzadeh, M., H. A. Najafabadi, M. Hakim, M. Feilizadeh, M. Vossoughi, and D. Rashtchian (2016) Experimental study and thermodynamic modeling for determining the effect of non-polar solvent (hexane)/polar solvent (methanol) ratio and moisture content on the lipid extraction efficiency from Chlorella vulgaris. Bioresour. Technol. 201: 304–311.
Kates, M. (1972) Techniques of lipidology: isolation, analysis, and identification of lipids. pp. 241–244. North-Holland Pub. Co., Amsterdam, Netherlands.
Guckert, J. B., K. E. Cooksey, and L. L. Jackson (1988) Lipid solvent systems are not equivalent for analysis of lipid classes in the microeukaryotic green alga, Chlorella. J. Microbiol. Meth. 8: 139–149.
Nagle, N. and P. Lemke (1990) Production of methyl ester fuel from microalgae. Appl. Biochem. Biotechnol. 24: 355–361.
Grima, E. M., A. R. Medina, A. G. Giménez, J. S. Pérez, F. G. Camacho, and J. G. Sánchez (1994) Comparison between extraction of lipids and fatty acids from microalgal biomass. J. Am. Oil Chem. Soc. 71: 955–959.
Fajardo, A. R., L. E. Cerdan, A. R. Medina, F. G. A. Fernández, P. A. G. Moreno, and E. M. Grima (2007) Lipid extraction from the microalga. Phaeodactylum tricornutum. Eur. J. Lipid. Sci. Technol. 109: 120–126.
Wayback Machine, Dielectric constant. http://macro.lsu.edu/HowTo/solvents/Dielectric%20Constant%20.htm
Folch, J., M. Lees, and G. Sloane-Stanley (1957) A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226: 497–509.
Bligh, E. G. and W. J. Dyer (1959) A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37: 911–917.
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Shin, HY., Shim, SH., Ryu, YJ. et al. Lipid Extraction from Tetraselmis sp. Microalgae for Biodiesel Production Using Hexane-based Solvent Mixtures. Biotechnol Bioproc E 23, 16–22 (2018). https://doi.org/10.1007/s12257-017-0392-9
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DOI: https://doi.org/10.1007/s12257-017-0392-9