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Expression Procedure Optimization of Carassius aurantus CYP1A in Shewanella Using Plasmid Construction Strategy

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An Erratum to this article was published on 01 March 2018

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Abstract

Cytochrome P4501A (CYP1A) has attracted an increasing interest due to its important role in metabolism of contaminants in aquatic environment and kinds of biomarkers to monitor the pollutants. CYPs are reported to express in E. coli, yeast and insect cells, while expression levels in these systems are too low to continue further study, such as functional and structural research. In this study, we construct an expression system using Shewanella oneidensis to produce goldfish CYP1A. RBS sequence that can elevate expression level by initiating the translation was added. A leading signal peptide which will direct the goal protein into periplasm of the host was introduced. Moreover, large-size plasmid construction strategy was applied during the successful construction process of expression system. At the position of ~60 kDa, a single band was seen clearly after expression; furthermore the amount of expressed CYP1A was as high as 0.02 micromoles per liter in the culture. Heme test was also performed, the result showed the typical P450 hemoprotein spectra. All these data suggest the possible suitable expression system for fish P4501A system was constructed.

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Change history

  • 12 May 2018

    In the 2018 issue of Biotechnology and Bioprocess Engineering (BBE), an error occurred in the research article: Yunqi Ma, Ming Lu, Ziwei Chang, Chu Lee, Hae-Kyun Yoo, and Jang-Su Park (2018) Expression Procedure Optimization of <Emphasis Type="Italic">Carassius aurantus</Emphasis> CYP1A in <Emphasis Type="Italic">Shewanella</Emphasis> Using Plasmid Construction Strategy. <Emphasis Type="Italic">Biotechnol. Bioprocess Eng</Emphasis>. 23: 86-92.

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Author notes

  1. These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Chemistry and Graduate School of Social Enterprise, Pusan National University, Busan, 609-735, Korea

    Yunqi Ma, Ming Lu, Ziwei Chang & Jang-Su Park

  2. East Sea Fisheries Research Institute, National Fisheries Research and Development Institute, Gangnenung, 210-861, Korea

    Chu Lee & Hae-Kyun Yoo

Authors
  1. Yunqi Ma
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  2. Ming Lu
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Correspondence to Jang-Su Park.

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Ma, Y., Lu, M., Chang, Z. et al. Expression Procedure Optimization of Carassius aurantus CYP1A in Shewanella Using Plasmid Construction Strategy. Biotechnol Bioproc E 23, 86–92 (2018). https://doi.org/10.1007/s12257-017-0330-x

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  • DOI: https://doi.org/10.1007/s12257-017-0330-x

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