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Hydrolytic activities of hydrolase enzymes from halophilic microorganisms

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Abstract

Biomass is normally processed using acidic or basic catalysts, which both have their drawbacks. One suitable alternative is the application of hydrolytic enzymes that can convert biomass into simpler molecules, which can be fermented and processed into biofuel. Hydrolytic enzymes include proteases, lipases, amylases, cellulases, mannanases, chitinases, and xylanases. To discover sources of these enzymes, 19 halophilic strains of microorganisms that are significantly resistant to high salt concentrations were analyzed. The objective of this research was to identify halophilic microorganisms that produce the target enzymes with high activities, and to characterize these enzymes according to their salt tolerances. The results obtained indicated that Pseudolateromonas phenolica, Micrococcus luteus, Pseudoalteromonas peptidolytica, Halomonas socia, Marinobacter maritimus, and Exiguobacterium aurantiacum strain 2 produced the highest protease, lipase, amylase, cellulase, mannanase, chitinase, and xylanase relative activities, respectively. Except for protease from P. phenolica, all the enzymes tested for salt resistance either maintained or increased their activities with increasing NaCl concentration.

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Authors and Affiliations

  1. Department of Environmental Engineering, College of Engineering, Ajou University, Suwon, Korea

    Jervian Johnson, Pamidimarri D. V. N. Sudheer & Kwon-Young Choi

  2. Department of Microbial Engineering, College of Engineering, Konkuk University, Seoul, Korea

    Yung-Hun Yang

  3. Department of Chemical Engineering, College of Engineering, Soongsil University, Seoul, Korea

    Yun-Gon Kim

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  1. Jervian Johnson
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  2. Pamidimarri D. V. N. Sudheer
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  5. Kwon-Young Choi
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Correspondence to Kwon-Young Choi.

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Johnson, J., Sudheer, P.D.V.N., Yang, YH. et al. Hydrolytic activities of hydrolase enzymes from halophilic microorganisms. Biotechnol Bioproc E 22, 450–461 (2017). https://doi.org/10.1007/s12257-017-0113-4

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