Effects of iron sources on the growth and lipid/carbohydrate production of marine microalga Dunaliella tertiolecta
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The effects of iron sources with different speciation and anionic moieties (ferric chloride, ferrous chloride, ferric EDTA, ferrous EDTA, ferric ammonium sulfate, and ferrous ammonium sulfate) on the cell growth and the production of energy storage (lipid and carbohydrate) from Dunaliella tertiolecta were investigated. The influence of iron dosage was also compared in the range from 0.65 mg/L (1X) to 6.5 mg/L (10X) as Fe concentration. Best cell growth rate was achieved when ferrous ammonium sulfate was used. Ferric EDTA resulted in higher lipid content than other iron sources, while ferrous ammonium sulfate favored the accumulation of carbohydrate among six iron sources. The accumulations of lipid and carbohydrate as energy storage competed each other and thus both contents did not increase together. In the presence of ferric EDTA, lipid content is increasing, while carbohydrate content is decreasing. On the contrary, lipid content is decreasing while carbohydrate is increasing in the presence of ferric ammonium sulfate. Because the overall carbohydrate content was larger than that of lipid, bioethanol production would be more advantageous than biodiesel production with the present D. tertiolecta strain if the carbohydrate in D. tertiolecta contains a high fraction of glucose with a good saccharification yield.
Keywordsmicroalgae Dunaliella tertiolecta iron cell growth lipid carbohydrate
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- 4.Kim, J. and J.-Y. Lee (2016) Enhanced autotrophic growth of Nannochloris sp. with trona buffer for sustainable carbon recycle. Bioetchnol. Bioproc. Eng. 10: 422–429.Google Scholar
- 5.Siaut, M., S. Cuiné, C. Cagnon, B. Fessler, M. Nguyen, P. Carrier, A. Beyly, F. Beisson, C. Triantaphylidès, Y. Li-Beisson, and G. Peltier (2011) Oil accumulation in the model green alga Chlamydomonas reinhardtii: Characterization, variability between common laboratory strains and relationship with starch reserves. BMC Biotechnol. 11: 7.CrossRefGoogle Scholar
- 13.Li, Y., Y. F. Chen, P. Chen, M. Min, W. Zhou, B. Martinez, J. Zhu, and R. Ruan (2011) Characterization of a microalga Chlorella sp. well adapted to highly concentrated municipal wastewater for nutrient removal and biodiesel production. Bioresour. Technol. 102: 5138–5144.Google Scholar
- 16.Mata, T. M., R. Almeida, and N. S. Caetano (2013) Effect of the culture nutrients on the biomass and lipid productivities of microalgae Dunaliella tertiolecta. Chem. Eng. Trans. 32: 973–978.Google Scholar
- 18.Guillard, R. R. L (1975) Culture of phytoplankton for feeding marine invertebrates. pp. 26-60. In Smith, W. L. and M. H. Chanley (eds.) Culture of Marine Invertebrate Animals. Plenum Press, NY, USA.Google Scholar
- 24.Sun, X., Y. Cao, H. Xu, Y. Liu, J. Sun, D. Qiao, and Y. Cao (2014) Effect of nitrogen-starvation, light intensity and iron on triacylglyceride/carbohydrate production and fatty acid profile of Neochloris oleoabundans HK-129 by a two-stage process. Bioresour. Technol. 155: 204–212.CrossRefGoogle Scholar
- 27.Sakthivel, R., S. Elumalai, and M. M. Arif (2011) Microalgae lipid research, past, present: A critical review for biodiesel production, in the future. J. Exp. Sci. 2: 29–49.Google Scholar
- 29.Kim, G., G. Mujtaba, and K. Lee (2016) Effects of nitrogen sources on cell growth and biochemical composition of marine chlorophyte Tetraselmis sp. for lipid production. Algae 31: 257–266.Google Scholar