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TB trifusion antigen adsorbed on calcium phosphate nanoparticles stimulates strong cellular immunity in mice

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Abstract

Among various vaccine candidates, subunit vaccines play an important role in immune protection against tuberculosis (TB). Calcium phosphate (CP) is considered as a strong inorganic adjuvant due to its great potential in increasing immune responses. The purpose of this study was to evaluate specific immune responses following the administration of trifusion-CP nanoparticles. The physiochemical properties of these nanoparticles, including morphology, particle size, zeta potential and adsorption rate, were measured in vitro. Subcutaneous immunization was performed three times on days 0, 14, and 28. Two weeks after the last administration, IFN-gamma, IL-4, and TGF-beta levels were measured by indirect enzyme linked immunosorbent assay (ELISA). The trifusion protein was successfully adsorbed onto calcium phosphate nanoparticles. The mean sizes of the resultant trifusion- CPN and CPN were 97.84 ± 12.08 and 67 ± 11.85 nm, respectively. CPN containing trifusion had stronger ability to induce IFN-gamma than the control groups. IL-4 and TGF-beta secretions in trifusion and trifusion-CPN groups were higher than those in the PBS group. However, there was no significant (p > 0.05) difference in IL-4 and TGFbeta concentrations between trifusion group and trifusion- CPN group. Therefore, calcium phosphate nanoparticles are good candidates for immunization against TB because antigen can be easily adsorbed onto CPN and strong cellular immune responses against CPN-antigen can be stimulated.

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Authors and Affiliations

  1. Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

    Yousef Amini & Mahdi Fasihi-Ramandi

  2. Microbiology & Virology Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran

    Bagher Moradi, Zahra Meshkat & Kiarash Ghazvini

  3. Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

    Mohsen Tafaghodi

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  1. Yousef Amini
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  2. Bagher Moradi
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  6. Mahdi Fasihi-Ramandi
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Correspondence to Mahdi Fasihi-Ramandi.

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Amini, Y., Moradi, B., Tafaghodi, M. et al. TB trifusion antigen adsorbed on calcium phosphate nanoparticles stimulates strong cellular immunity in mice. Biotechnol Bioproc E 21, 653–658 (2016). https://doi.org/10.1007/s12257-016-0326-y

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  • DOI: https://doi.org/10.1007/s12257-016-0326-y

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