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In vivo fluorescence imaging to assess early therapeutic response to tumor progression in a xenograft cancer model

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Abstract

Bioimaging technology plays an important role in assessment of therapeutic response in cancer therapy. Here, we report a non-invasive monitoring system for measuring tumor growth by in vivo fluorescence imaging. Target cells for xenograft tumor induction were manipulated by cell-surface fluorescence labelling. Fluorescence was clearly detected in vitro and in vivo without affecting cytotoxicity. Anti-tumor efficacy was evaluated by directly measuring the fluorescence signal of a progressive tumor in a xenograft model. This non-invasive in vivo monitoring system can be used to assess the early response to antitumor therapeutics and may be a valuable tool to replace or complement traditional caliper-based methods for preclinical studies.

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Authors and Affiliations

  1. Bioimaging Research Team, Korea Basic Science Institute, Cheongju, 28119, Korea

    Eun-Joong Kim, Hyunseung Lee, Areum Yeom & Kwan Soo Hong

  2. Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, 34134, Korea

    Kwan Soo Hong

Authors
  1. Eun-Joong Kim
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  2. Hyunseung Lee
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  3. Areum Yeom
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  4. Kwan Soo Hong
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Correspondence to Kwan Soo Hong.

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These authors equally contributed to this work.

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Kim, EJ., Lee, H., Yeom, A. et al. In vivo fluorescence imaging to assess early therapeutic response to tumor progression in a xenograft cancer model. Biotechnol Bioproc E 21, 567–572 (2016). https://doi.org/10.1007/s12257-016-0251-0

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  • DOI: https://doi.org/10.1007/s12257-016-0251-0

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