Biotechnology and Bioprocess Engineering

, Volume 21, Issue 5, pp 634–640 | Cite as

Isomerase activity of Candida rugosa lipase in the optimized conversion of racemic ibuprofen to (S)-ibuprofen

  • Saideh S. Mortazavi
  • David Chavez-Flores
  • James M. Salvador
Research Paper

Abstract

The Candida rugosa lipase catalyzed Dynamic Kinetic Resolution of racemic ibuprofen methyl ester produced (S)-ibuprofen in over 90% yield within 72 h at pH 7.6. The best concentration of various buffers for these reactions ranged from 0.2 to 0.5 M. The commercial lipase was found to be acidic altering the final pH of the reaction mixtures. Dimethylformamide co-solvent maintained the reaction pH better than dimethylsulfoxide. Lower concentrations of ibuprofen methyl ester and higher stirring rates led to faster conversions. The minimal amount of lipase needed was 20 mg/mL buffer. Reaction of (R)-ibuprofen methyl ester under the optimized conditions excluding the lipase led to no racemization, indicating that the conversion of (R)-ibuprofen methyl ester to (S)-ibuprofen is catalyzed by the enzyme, thus, indicating Candida rugosa lipase possess Isomerase activity.

Keywords

dynamic kinetic resolution Candida rugosa lipase ibuprofen methyl ester (S)-Ibuprofen racemization dimethylformamide 

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Copyright information

© The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Saideh S. Mortazavi
    • 1
  • David Chavez-Flores
    • 2
  • James M. Salvador
    • 1
  1. 1.Department of ChemistryUniversity of Texas at El PasoEl PasoUSA
  2. 2.Facultad de Ciencias QuímicasUniversidad Autónoma de ChihuahuaChihuahuaMexico

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