Abstract
Chitosan is an available natural polysaccharide that is biocompatible, biodegradable, and non-toxic. In this study, to enhance the antibacterial activity of water-soluble chitosan (WSC), amino acids with hydrophobic, anionic, or cationic side chains were grafted to the amine groups of WSC (amino acid-grafted WSC; AGW). Chemical characterization of AGW was performed by 1H nuclear magnetic resonance spectroscopy, resulting in grafting molecular percentages of 7.3 ~ 9.9%. AGW exhibited more potent antibacterial activity than unmodified WSC in both drug-susceptible and drug-resistant bacteria. Scanning electron microscopy and a calcein leakage assay in bacteria and artificial liposomes, respectively, revealed the antibacterial action of AGW to be membranolytic. Lysine or isoleucinegrafted WSC showed potent in vivo antibacterial activity in a drug-resistant Pseudomonas aeruginosa-infected mouse model. AGW has potential use as an effective antibacterial agent.
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03 September 2019
In the 2016 issue of Biotechnology and Bioprocess Engineering (BBE), an error occurred in the research article: Jun-Ho Kim, Nam-Hong Kim, Eun-Ji Kim, Ji Ho Kim, Min-Young Lee, Yung-Hoon Park, Jung Ro Lee, Seong-Cheol Park, and Mi-Kyeong Jang (2016) Antibacterial Effects of Amino Acids-grafted Water-soluble Chitosan against Drug-resistant Bacteria. Biotechnol. Bioprocess Eng. 21: 183���189.
12 July 2021
An Erratum to this paper has been published: https://doi.org/10.1007/s12257-016-2144-7
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Kim, JH., Kim, NH., Kim, EJ. et al. Antibacterial effects of amino acids-grafted water-soluble chitosan against drug-resistant bacteria. Biotechnol Bioproc E 21, 183–189 (2016). https://doi.org/10.1007/s12257-016-0144-2
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DOI: https://doi.org/10.1007/s12257-016-0144-2