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A rapidly new-typed detection of norovirus based on F0F1-ATPase molecular motor biosensor

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Abstract

In order to adapt port rapid detection of food borne norovirus, presently we developed a new typed detection method based on F0F1-ATPase molecular motor biosensor. A specific probe was encompassed the conservative region of norovirus and F0F1-ATPase within chromatophore was constructed as a molecular motor biosensor through the “ε-subunit antibody-streptomycin-biotin-probe” system. Norovirus was captured based on probe-RNA specific binding. Our results demonstrated that the Limit of Quantification (LOQ) is 0.005 ng/mL for NV RNA and also demonstrated that this method possesses specificity and none cross-reaction for food borne virus. What’s more, the experiment used this method could be accomplished in 1 h. We detected 10 samples by using this method and the results were consistent with RT-PCR results. Overall, based on F0F1-ATPase molecular motors biosensor system we firstly established a new typed detection method for norovirus detection and demonstrated that this method is sensitive and specific and can be used in the rapid detection for food borne virus.

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Authors and Affiliations

  1. Technical Center for Safety of Industrial Products, Tianjin Entry-Exit Inspection Quarantine Bureau, Tianjin, 300-308, China

    Zhuo Zhao, Zhi-Peng Liu, Hua Wang, Ming Liu, Yan-Yan Yu, Li Sun & Hui Zhang

  2. Beijing Entry-Exit Inspection Quarantine Bureau, Beijing, 100-026, China

    Jie Zhang

  3. Linyi Entry-Exit Inspection Quarantine Bureau, Linyi, 276-034, China

    Mei-Ling Xu

  4. Weifang people’s hospital of high-tech industrial development zone, Weifang, 261-205, China

    Hai-Yan Wu

Authors
  1. Zhuo Zhao
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  2. Jie Zhang
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  3. Mei-Ling Xu
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  4. Zhi-Peng Liu
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  5. Hua Wang
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  6. Ming Liu
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  7. Yan-Yan Yu
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  8. Li Sun
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  9. Hui Zhang
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  10. Hai-Yan Wu
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Correspondence to Jie Zhang.

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These authors contributed equally to this work.

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Zhao, Z., Zhang, J., Xu, ML. et al. A rapidly new-typed detection of norovirus based on F0F1-ATPase molecular motor biosensor. Biotechnol Bioproc E 21, 128–133 (2016). https://doi.org/10.1007/s12257-015-0384-6

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  • DOI: https://doi.org/10.1007/s12257-015-0384-6

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