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Dissimilatory azoreduction of Orange I by a newly isolated moderately thermophilic bacterium, Novibacillus thermophilus SG-1

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Abstract

Dye wastewater normally is discharged at high temperature, but thermophilic bacteria capable of decolorizing azo dyes have rarely been isolated. Here we report a newly isolated moderately thermophilic bacterium, Novibacillus thermophilus SG-1, which had a remarkable ability to decolorize the azo dye Orange I by utilizing a large variety of organic substrates as electron donors. When Orange I served as the sole electron acceptor, almost complete decolorization occurred at 50ºC and pH 8.0 with acetate as the electron donor after anaerobic incubation of strain SG-1 for 24 h. The decolorization process followed the pseudofirst- order kinetics. The complete reduction of 0.3 mM Orange I was accompanied by a stoichiometric consumption of 0.17 mM acetate over time. The measured molar ratio (1.76) of Orange I reduced to acetate oxidized was close to the theory value of 2.0, suggesting that most of the electrons released by acetate had been transported to Orange I. Simultaneously energy generated from the electron transfer process was used to support cell anaerobic growth, which meant that azoreduction by strain SG-1 is an azorespiration process. To our knowledge, this is the first report of a thermophilic bacterium capable of azorespiration, which increases the limited number of bacteria for treating hightemperature azo dye wastewater.

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Authors and Affiliations

  1. Guangdong Key Laboratory of Agricultural Environment Pollution Integrated Control, Guangdong Institute of Eco-Environment and Soil Sciences, Guangzhou, 510-650, China

    Zhen Yu, Yueqiang Wang, Guiqin Yang & Shungui Zhou

  2. 101 Research Institute of the Ministry of Civil Affairs, Beijing, 100-070, China

    Xuemei Zhou

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  1. Zhen Yu
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  2. Xuemei Zhou
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  3. Yueqiang Wang
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  4. Guiqin Yang
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  5. Shungui Zhou
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Correspondence to Yueqiang Wang.

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Yu, Z., Zhou, X., Wang, Y. et al. Dissimilatory azoreduction of Orange I by a newly isolated moderately thermophilic bacterium, Novibacillus thermophilus SG-1. Biotechnol Bioproc E 20, 1064–1070 (2015). https://doi.org/10.1007/s12257-015-0365-9

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  • DOI: https://doi.org/10.1007/s12257-015-0365-9

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