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Production and characterization of bacterial cellulose by Leifsonia sp. CBNU-EW3 isolated from the earthworm, Eisenia fetida

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Abstract

A total of five bacterial strains were isolated from earthworm, Eisenia fetida and examined for bacterial cellulose (BC) production in Hestrin-Schramm medium (HS). Among the five strains tested, CBNU-EW3 exhibited excellent BC production and was identified as Leifsonia sp. by 16S rDNA sequence analysis. BC production by Leifsonia sp. CBNU-EW3 was optimum at pH 5, 30°C, and with glucose and yeast extract as carbon and nitrogen sources, respectively, according to 15 day-long experiments. (XRD) analysis of the dried pellicle indicated that the BC was partially crystalline type I. Fourier transform infrared spectroscopy (FT-IR) analysis showed that the obtained pellicle contained the same functional groups as typical BC. Field emission scanning electron microscopy (FESEM) images showed that the BC micro-fibril matrix consisted of a flat surface with large pore size and cellulose aggregation.

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Authors and Affiliations

  1. Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, 570-752, Korea

    Palanivel Velmurugan, Muthusamy Govarthanan, Young-Joo Yi & Byung-Taek Oh

  2. Department of Ecology Landscape Architecture–Design, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, 570-752, Korea

    Hyun Myung

  3. Korea Rural Community Corp., Naju, 520-350, Korea

    Sang-Ki Seo

  4. National Institute of Crop Science, RDA, Iksan, 570-080, Korea

    Kwang-Min Cho

  5. USDA-ARS, AWMRU, Bowling Green, 230 Bennett Lane, KY, 42104, USA

    Nanh Lovanh

  6. Plant Medical Research Center, College of Agricultural and Life Sciences, Chonbuk National University, Jeonju, 561-756, Korea

    Byung-Taek Oh

Authors
  1. Palanivel Velmurugan
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  2. Hyun Myung
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Correspondence to Byung-Taek Oh.

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Velmurugan, P., Myung, H., Govarthanan, M. et al. Production and characterization of bacterial cellulose by Leifsonia sp. CBNU-EW3 isolated from the earthworm, Eisenia fetida . Biotechnol Bioproc E 20, 410–416 (2015). https://doi.org/10.1007/s12257-014-0793-y

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  • DOI: https://doi.org/10.1007/s12257-014-0793-y

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