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Recombinant mussel coating protein fused with cell adhesion recognition motif enhanced cell proliferation

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Abstract

Endurable coating on biomedical substrates is one of the most important issues in biomedical engineering field. Recently, a robust yet reversible coating has found in the cuticle of mussel byssus and the mimicking of the mussel cuticle coating has been considered as a strategy to build up endurable coating on biomedical materials. To date, the only known protein in the cuticle is mussel foot protein-1 (fp-1). To form endurable and bioactive coating on the biomedical surface, the fusion protein of fp-1 and GRGDSP peptide (fp-1-RGD) was genetically designed and produced in E. coli. The fusion protein of fp-1-RGD was successfully expressed as a form of inclusion body and was simply purified by diluted acetic acid extraction with high purity (~95%). Fp-1-RGD was coated on the tissue culture polystyrene (TCPS) and showed better preosteoblast cell proliferation than that of TCPS. Therefore, the marriage of fp-1 and bioactive peptide can be a good strategy to form bioactive and endurable coating in biomedical field.

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Authors and Affiliations

  1. Integrative Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang, 790-784, Korea

    Hee Young Yoo & Dong Soo Hwang

  2. School of Chemical Engineering, Yeungnam University, Gyeongsan, 712-749, Korea

    Young Hoon Song & Jeong Hyun Seo

  3. Department of Chemical Engineering, Pohang University of Science and Technology, Pohang, 790-784, Korea

    Hyung Joon Cha

  4. School of Environmental Science and Engineering, Pohang University of Science and Technology, Pohang, 790-784, Korea

    Dong Soo Hwang

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  1. Hee Young Yoo
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Correspondence to Dong Soo Hwang.

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Yoo, H.Y., Song, Y.H., Seo, J.H. et al. Recombinant mussel coating protein fused with cell adhesion recognition motif enhanced cell proliferation. Biotechnol Bioproc E 20, 211–217 (2015). https://doi.org/10.1007/s12257-014-0621-4

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  • DOI: https://doi.org/10.1007/s12257-014-0621-4

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