Biotechnology and Bioprocess Engineering

, Volume 20, Issue 2, pp 211–217 | Cite as

Recombinant mussel coating protein fused with cell adhesion recognition motif enhanced cell proliferation

  • Hee Young Yoo
  • Young Hoon Song
  • Jeong Hyun Seo
  • Hyung Joon Cha
  • Dong Soo Hwang
Research Paper


Endurable coating on biomedical substrates is one of the most important issues in biomedical engineering field. Recently, a robust yet reversible coating has found in the cuticle of mussel byssus and the mimicking of the mussel cuticle coating has been considered as a strategy to build up endurable coating on biomedical materials. To date, the only known protein in the cuticle is mussel foot protein-1 (fp-1). To form endurable and bioactive coating on the biomedical surface, the fusion protein of fp-1 and GRGDSP peptide (fp-1-RGD) was genetically designed and produced in E. coli. The fusion protein of fp-1-RGD was successfully expressed as a form of inclusion body and was simply purified by diluted acetic acid extraction with high purity (~95%). Fp-1-RGD was coated on the tissue culture polystyrene (TCPS) and showed better preosteoblast cell proliferation than that of TCPS. Therefore, the marriage of fp-1 and bioactive peptide can be a good strategy to form bioactive and endurable coating in biomedical field.


biomedical coating material mussel coating protein RGD peptide cell recognition motif fusion protein 


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Copyright information

© The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Hee Young Yoo
    • 1
  • Young Hoon Song
    • 2
  • Jeong Hyun Seo
    • 2
  • Hyung Joon Cha
    • 3
  • Dong Soo Hwang
    • 1
    • 4
  1. 1.Integrative Bioscience and BioengineeringPohang University of Science and TechnologyPohangKorea
  2. 2.School of Chemical EngineeringYeungnam UniversityGyeongsanKorea
  3. 3.Department of Chemical EngineeringPohang University of Science and TechnologyPohangKorea
  4. 4.School of Environmental Science and EngineeringPohang University of Science and TechnologyPohangKorea

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