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Nitro-oleic acid decreases transcription of the angiotensin II type I receptor gene in aortic smooth muscle cells

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An Erratum to this article was published on 01 April 2015

Abstract

Nitroalkene derivatives of nitro-oleic acid (OA-NO2) regulate pluripotent cell signaling in vivo under physiological and pathological conditions. Angiotensin II type 1 receptor (AT1R) plays an important role in the cardiovascular system. In this study, OA-NO2 reduced the AT1R mRNA level, specifically in primary smooth muscle cells, showing a 70% reduction in rat smooth muscle cells (RASMCs) and a 50% reduction in pig smooth muscle cells (PASMCs). These effects were not observed in CHO cells, which highly express AT1R. The AT1R mRNA decay rate was unchanged after OA-NO2 compared with OA treatment. Nitric oxide (NO) and peroxisome proliferator-activated receptor gamma (PPARγ) did not alter the reduced effects of OA-NO2 on the AT1R mRNA level in SMCs. However, Sp1-mediated activation of the AT1R promoter was reduced in response to OA-NO2 in RASMCs but not 293T cells. In addition, the nuclear factor-kappa B (NF-κB) pathway was involved in the OA-NO2-mediated downregulation of AT1R transcription in SMCs. Taken together, our results demonstrate that OA-NO2 specifically inhibits AT1R mRNA expression in primary smooth muscle cells via the NF-κB pathway.

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Authors and Affiliations

  1. Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Jilin, 130062, China

    Huan Wang, Hongsheng Ouyang, Zhuang Li, Xiaolei Han, Xingxing Liu, Guangyao Ran, Gangqi Wang, Daxin Pang & Xiaochun Tang

  2. Department of Dermatology and Venerology, 1st Hospital, Jilin University, Jilin, 130062, China

    Yaping Tian

  3. Department of Bioengineering, Collage of Jilin Agricultural Science and Technology, Jilin University, Jilin, 130062, China

    Huan Wang

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  1. Huan Wang
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  2. Hongsheng Ouyang
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  4. Zhuang Li
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  5. Xiaolei Han
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  10. Xiaochun Tang
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Correspondence to Xiaochun Tang.

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Wang, H., Ouyang, H., Tian, Y. et al. Nitro-oleic acid decreases transcription of the angiotensin II type I receptor gene in aortic smooth muscle cells. Biotechnol Bioproc E 19, 740–746 (2014). https://doi.org/10.1007/s12257-014-0255-6

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  • DOI: https://doi.org/10.1007/s12257-014-0255-6

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