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Molecular cloning and characterization of a novel glucansucrase from Leuconostoc mesenteroides subsp. mesenteroides LM34

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Abstract

Leuconostoc mesenteroides LM34 was isolated from kimchi, a traditional fermented Korean food. L. mesenteroides LM34 produced extracellular glucansucrase (DSRLM34), which is responsible for the synthesis of soluble glucan using sucrose. The DSRLM34 gene consists of a 4,503 bp open reading frame (ORF) and encodes an enzyme of 1,500 amino acids with an apparent molecular mass of 165 kDa. The deduced amino-acid sequence showed the highest amino-acid sequence identity (98%) to that of glucansucrase of Lactobacillus lactis. The gene was over-expressed in Escherichia coli strain and the recombinant enzyme (rDSRLM34) was purified. Both DSRLM34 and rDSRLM34 synthesized glucan mainly containing α-1, 6 glucosidic linkage and branched α-1, 3 glucosidic linkages. The enzyme exhibited optimum activity at 30°C and pH 5.0. DSRLM34 has promising potential as a thickening agent in sucrose-supplemented milk.

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Authors and Affiliations

  1. Department of Biomedical Science, Chosun University, Gwangju, 501-759, Korea

    Hee-Kyoung Kang

  2. Institutes of Green Bio Science & Technology, Seoul National University, Pyeongchang, 232-916, Korea

    Thi Thanh Hanh Nguyen, Ha-Na Jeong, Min-Eon Park & Doman Kim

  3. Department of Food and Animal Biotechnology, College of Agriculture and Life Science, Seoul National University, Seoul, 151-742, Korea

    Doman Kim

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  1. Hee-Kyoung Kang
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  2. Thi Thanh Hanh Nguyen
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  5. Doman Kim
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Correspondence to Doman Kim.

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Kang, HK., Nguyen, T.T.H., Jeong, HN. et al. Molecular cloning and characterization of a novel glucansucrase from Leuconostoc mesenteroides subsp. mesenteroides LM34. Biotechnol Bioproc E 19, 605–612 (2014). https://doi.org/10.1007/s12257-014-0116-3

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  • DOI: https://doi.org/10.1007/s12257-014-0116-3

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