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A comparative proteomic analysis of parthenogenetic lines and amphigenetic lines of silkworm

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Abstract

Parthenogenetic strains of silkworm serve as an effective system for sex-control in silkworms. To determine the molecular mechanism of silkworm parthenogenesis, protein profiles from newly hatched silkworm of a parthenogenetic lines with high pigmentation rate and hatching rate were compared with amphigenetic lines using proteomics approach, including by two-dimensional electrophoresis (2-DE), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/TOF MS), and bioinformatics analysis. Several proteins were expressed differentially between the parthenogenetic and amphigenetic lines, and seven of nine interesting proteins were identified successfully using MALDI-TOF/TOF MS analysis. The identified proteins were muscular protein-20, odorant binding protein-LOC100301497, glutathione S-transferase delta, translationally controlled tumor protein homolog, cuticular protein RR-1 motif 19, beta-actin, actins, and muscle-type A1 actins. These proteins may be associated with the regulation of growth, development, and reproductive processes of silkworm parthenogenetic lines.

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Authors and Affiliations

  1. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210-095, China

    Peigang Liu & Fangxiong Shi

  2. Sericulture Research Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310-021, China

    Peigang Liu, Yongqiang Wang, Xin Du & Zhiqi Meng

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Correspondence to Fangxiong Shi.

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Liu, P., Wang, Y., Du, X. et al. A comparative proteomic analysis of parthenogenetic lines and amphigenetic lines of silkworm. Biotechnol Bioproc E 19, 641–649 (2014). https://doi.org/10.1007/s12257-014-0099-0

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