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Enhanced catalytic ability of Candida rugosa lipase immobilized on pore-enlarged hollow silica microspheres and cross-linked by modified dextran in both aqueous and non-aqueous phases

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Abstract

Candida rugosa lipase (CRL) is one of the most widely used lipases. To enhance the catalytic abilities of CRL in both aqueous and non-aqueous phases, hollow silica microspheres (HSMSs) with a pore size of 18.07 nm were used as an immobilization support, and aldehydecontaining dextrans were employed to further cross-link the adsorbed CRL. In the experimental ranges examined, the loading amount of lipase linearly increased to 171 ± 3.4 mgprotein/gsupport with the CRL concentration and all the adsorption equilibriums were reached within 30 min. After simple cross-linking, the tolerance to pH 4.0 ∼ 8.0 as well as the thermal stability of immobilized CRL at 40 ∼ 80°C were both substantially increased, and 82 ± 2.1% activity remaining after the sixth reuse. The immobilized CRL was successfully applied to the resolution of racemic ibuprofen in non-aqueous phase. The initial reaction rate increased by 1.4- and 3.6-fold compared with the rates of adsorbed and native lipases, respectively. Furthermore, the R-ibuprofen was obtained at ee > 93%, and the enantiomeric ratio reached E > 140 at the conversion of 50 ± 1.5% within 48 h.

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Authors and Affiliations

  1. College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, 211-816, China

    Liwei Ren, Honghua Jia, Min Yu, Hua Zhou & Ping Wei

  2. Key Laboratory of Carbon Materials, Institute of Coal Chemistry, Chinese Academy Sciences, Taiyuan, 030-001, China

    Wenzhong Shen

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  1. Liwei Ren
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  2. Honghua Jia
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  3. Min Yu
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  4. Wenzhong Shen
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  5. Hua Zhou
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  6. Ping Wei
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Correspondence to Hua Zhou.

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Ren, L., Jia, H., Yu, M. et al. Enhanced catalytic ability of Candida rugosa lipase immobilized on pore-enlarged hollow silica microspheres and cross-linked by modified dextran in both aqueous and non-aqueous phases. Biotechnol Bioproc E 18, 888–896 (2013). https://doi.org/10.1007/s12257-013-0044-7

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