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Preparation and evaluation of a peptide nucleic acid gene chip system associated with surface plasmon resonance

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Abstract

The aim of this study was to construct a gene chip system based on a surface plasmon resonance technique, where peptide nucleic acid (PNA) oligomers are used as probes. Since the self-assembled monolayer (SAM) technology offers good control at the molecular level, we prepared 2D surface chemistry via SAM for probe attachments. PNA, which was designed according to the bioinformatics, was immobilized on the SAM-modified chip, and subsequently, relevant parameters of the experiment were ensured and optimized. Our results suggest that the ion strength and pH value of the buffer solution do not play significant roles in PNA or its complementary strand hybridization. The PNA probe binds to its complementary nucleic acid strand with a higher sensitivity and specificity compared to those of a traditional DNA probe. The PNA probe combined with surface plasmon resonance (SPR) technology has the benefits of being a label-free and in-real time monitor, as well as having improved hybridization and stability efficiency, which highlight the PNA gene chip detection system as a promising biosensor for clinical applications.

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Authors and Affiliations

  1. Institute of Disease Control and Prevention, Shenzhen International Travel Health Care Center, Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen, China

    Qingye Ou, Jian’an He, Chunqiao Liu, Lei Shi, Chunzhong Zhao, Yungqing Xu & Dayong Gu

  2. Zhuhai Center for Chronic Disease Control, Zhuhai, China

    Qingye Ou

  3. School of electronics engineering and computer science, Peking University, Beijing, China

    Jian’an He

Authors
  1. Qingye Ou
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  2. Jian’an He
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  3. Chunqiao Liu
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  4. Lei Shi
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  5. Chunzhong Zhao
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  6. Yungqing Xu
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  7. Dayong Gu
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Corresponding author

Correspondence to Dayong Gu.

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These authors contributed equally to this work.

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Ou, Q., He, J., Liu, C. et al. Preparation and evaluation of a peptide nucleic acid gene chip system associated with surface plasmon resonance. Biotechnol Bioproc E 18, 1031–1037 (2013). https://doi.org/10.1007/s12257-012-0844-1

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  • DOI: https://doi.org/10.1007/s12257-012-0844-1

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