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Thermodynamics and kinetics of lysozyme adsorption onto two kinds of weak cation exchangers

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Abstract

The present work investigated the adsorption behaviors of lysozyme onto weak cation exchangers at different temperatures. The adsorption isotherm, adsorption thermodynamics and adsorption kinetics were studied. The results indicate that the adsorption of lysozyme onto acrylic acid copolymer based beads (Hydrolite D115) is spontaneous and exothermic, while that onto agarose based beads (CM Sepharose 6 Fast Flow) is also spontaneous, but endothermic. The pseudo second-order kinetic model fits well to the dynamic adsorption experimental data, and the kinetic results are also in concert with the adsorption thermodynamics.

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Authors and Affiliations

  1. School of Pharmaceutical Science, Jiangnan University, Wuxi, 214-122, China

    Yong-Mei Cheng & Jing-Hua Chen

  2. National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, 214-122, China

    Xiong-Hua Jin & Hai-Feng Xia

  3. Key Laboratory of Synthetic and Natural Functional Molecular Chemistry of Ministry of Education, Institute of Modern Separation Science, Northwest University, Shaanxi Key laboratory of Modern Separation Science, Xi’an, 710-068, China

    Dong Gao

  4. Zhejiang Hisun Pharmaceutical Co., Ltd., Taizhou, 318000, China

    Xiong-Hua Jin

Authors
  1. Yong-Mei Cheng
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  2. Xiong-Hua Jin
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  3. Dong Gao
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  4. Hai-Feng Xia
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  5. Jing-Hua Chen
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Correspondence to Hai-Feng Xia.

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These authors contributed equally to this work.

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Cheng, YM., Jin, XH., Gao, D. et al. Thermodynamics and kinetics of lysozyme adsorption onto two kinds of weak cation exchangers. Biotechnol Bioproc E 18, 950–955 (2013). https://doi.org/10.1007/s12257-012-0765-z

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  • DOI: https://doi.org/10.1007/s12257-012-0765-z

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