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Carbon dioxide injection method for enhancing hydrogenotrophic denitrification of secondary wastewater effluent in fixed bed reactor

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Abstract

This study presents an optimal injection method for using carbon dioxide as a carbon source for the hydrogenotrophic denitrification of secondary wastewater effluent in a laboratory-scale fixed bed reactor (FBR). The FBR was operated under three conditions: a continuous CO2 supply, periodic CO2 supply, and without a CO2 supply. The continuous operation of the FBR without carbon dioxide injection resulted in an increase in pH to 10 and a noticeable level of nitrite accumulation. The continuous co-injection of carbon dioxide and hydrogen gas decreased the pH to a range of 6 ∼ 8, but the denitrification efficiency decreased to 29%. The co-injection of carbon dioxide decreased the maximum dissolved hydrogen concentration and hydrogen mass transfer rate by 25 and 61%, respectively. Compared to the continuous injection method, a periodic injection of carbon dioxide increased the denitrification efficiency from 28.6 to 85% as the hydrogen flow rate and hydraulic retention time (HRT) increased. With the periodic injection of carbon dioxide, the nitrite accumulation appeared to be insignificant as the hydrogen flow rate increased.

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References

  1. Kim, J. H., J. S. Lee, G. B. Jung, S. G. Yun, M. H. Koh, J. C. Shim, and S. K. Kwun (2003) Improvement of water quality standards for Korean agriculture by comparison with foreign countries’ case. Kor. J. Int. Agricult. 15: 179–188.

    Google Scholar 

  2. Friedler, E. (1999) The Jeezrael valley project for wastewater reclamation and reuse. Water Sci. Technol. 40: 43–50.

    Google Scholar 

  3. Zulu, G., M. Toyota, and S. Misawa (1996) Characteristics of water reuse and its effects on paddy irrigation system water balance and the rice land ecosystem. Agricult. Wat. Manag. 31: 269–283.

    Article  Google Scholar 

  4. WHO (2004) Nitrates and nitrites in drinking-water. Rolling revision of the WHO guidelines for drinking-water quality. Draft for review and comments. World Health Organization, Geneva.

    Google Scholar 

  5. Xiong, J., G. Guo, Q. Mahmood, and M. Yue (2011) Nitrogen removal from secondary effluent by using integrated constructed wetland system. Ecologic. Eng. 37: 659–662.

    Article  Google Scholar 

  6. Kaneko, S. and K. Nakajima (1988) Phosphorus removal by crystallization using a granular activated magnesia clinker. J. Water Pollution Control Fed. 60: 1239–1244.

    CAS  Google Scholar 

  7. Hashimoto, S. and K. Furukawa (1989) Nutrient removal from secondary effluent by filamentous algae. J. Ferment. Bioeng. 67: 62–69.

    Article  CAS  Google Scholar 

  8. Kang, S. K., K. H. Choo, and K. H. Lim (2003) Use of iron oxide particles as adsorbents to enhance phosphorus removal from secondary wastewater effluent. Separation Sci. Technol. 38: 3853–3874.

    Article  CAS  Google Scholar 

  9. Kuroda, M., T. Watanabe, and Y. Umedu (1997) Simultaneous COD removal and denitrification of wastewater by bio-electro reactors. Water Sci. Technol. 35: 161–168.

    CAS  Google Scholar 

  10. Lee, C. K. and R. E. Rittmann (2002) Applying a novel autohydrogenotrophic hollow-fiber membrane biofilm reactor for denitrification of drinking water. Water Res. 36: 2040–2052.

    Article  CAS  Google Scholar 

  11. Schnobrich, M. R., B. P. Chaplin, M. J. Semmens, and P. J. Novak (2007) Stimulating hydrogenotrophic denitrification in simulated groundwater containing high dissolved oxygen and nitrate concentrations. Water Res. 41: 1869–1876.

    Article  CAS  Google Scholar 

  12. Ergas, S. J. and A. F. Reuss (2001) Hydrogenotrophic denitrification of drinking water using a hollow fibre membrane bioreactor. J. Water Supply. 50: 161–171

    CAS  Google Scholar 

  13. Karanasios, K. A., I. A. Vasiliadou, S. Pavlou, and D. V. Vayenas (2010) Hydrogenotrophic denitrification of potable water: A review. J. Hazardous Mat. 180: 20–37.

    Article  CAS  Google Scholar 

  14. Kurt, M., J. Dunn, and J. R. Bourne (1987) Biological denitrification of drinking water using autotrophic organisms with H2 in a fluidized-bed biofilm reactor. Biotechnol. Bioeng. 29: 493–501.

    Article  CAS  Google Scholar 

  15. Rezania, B., J. A. Oleszkiewicz, and N. Cicek (2007) Hydrogendependent denitrification of water in an anaerobic submerged membrane bioreactor coupled with a novel hydrogen delivery system. Water Res. 41: 1074–1080.

    Article  CAS  Google Scholar 

  16. Gros, H., G. Schnoor, and P. Rutten (1988) Biological denitrification process with hydrogen-oxidizing bacteria for drinking water treatment. Water Suppl. 6: 193–198.

    CAS  Google Scholar 

  17. Grommen, R., M. Verhaege, and W. Verstraete (2006) Removal of nitrate in aquaria by means of electrochemically generated hydrogen gas as electron donor for biological denitrification. Aquacultural Eng. 34: 33–39.

    Article  Google Scholar 

  18. Vasiliadou, I. A., K. A. Karanasios, S. Pavlou, and D. V. Vayenas (2009) Experimental and modeling study of drinking water hydrogenotrophic denitrification in packed-bed reactors. J. Hazardous Mat. 165: 812–824.

    Article  CAS  Google Scholar 

  19. Lee, J. W., K. H. Lee, K. Y. Park, and S. K. Maeng (2010) Hydrogenotrophic denitrification in a packed bed reactor: Effects of hydrogen to water flow rate ratio. Bioresour. Technol. 101: 3940–3946.

    Article  CAS  Google Scholar 

  20. Lee, C. K. and R. E. Rittmann (2003) Effects of pH and precipitation on hydrogenotrophic denitrification using the hollow-fiber membrane biofilm reactor. Water Res. 37: 1551–1556.

    Article  CAS  Google Scholar 

  21. Rezania, B., N. Cicek, and J. A. Oleszkiewicz (2005) Kinetics of hydrogen-dependent denitrification under varying pH and temperature conditions. Biotechnol. Bioeng. 92: 900–906.

    Article  CAS  Google Scholar 

  22. Ghafari, S., M. Hasan, and M. K. Aroua (2009) Effect of carbon dioxide and biocarbonate as inorganic carbon sources on growth and adaptation of autohydrogenotrophic denitrifying bacteria. J. Hazardous Mat. 162: 1507–1513.

    Article  CAS  Google Scholar 

  23. Ho, C. M., S. K. Tseng, and Y. J. Chang (2001) Autotrophic denitrification via a novel membrane-attached biofilm reactor. Appl. Microbiol. 33: 201–205.

    Article  CAS  Google Scholar 

  24. Celmer, D., J. Oleszkiewicz, N. Cicek, and H. Husain (2006) Hydrogen limitation — a method for controlling the performance of membrane biofilm reactor for autotrophic denitrification of wastewater. Water Sci. Technol. 54: 165–172.

    CAS  Google Scholar 

  25. Tziotzios, G., M. Teliou, V. Kaltsouni, G. Lyberatos, and D. V. Vayenas (2005) Biological phenol removal using suspended growth and packed bed reactors. Biochem. Eng. J.. 26: 65–71.

    Article  CAS  Google Scholar 

  26. Fontenot, Q., C. Bonvillain, M. Kilgen, and R. Boopathy (2007) Effects of temperature, salinity, and carbon: Nitrogen ratio on sequencing batch reactor treating shrimp aquaculture wastewater. Bioresour. Technol. 98: 1700–1703.

    Article  CAS  Google Scholar 

  27. Glass, C. and J. Silverstein (1998) Denitrification kinetics of high nitrate concentration water: pH effect on inhibition and nitrite accumulation. Water Res. 32: 831–839.

    Article  CAS  Google Scholar 

  28. Chang, C. C., S. K. Tseng, and H. K. Huang (1999) Hydrogenotrophic denitrification with immobillized Alcaligenes eutrophus for drinking water treatment. Bioresour. Technol. 69: 53–58.

    Article  CAS  Google Scholar 

  29. Fang, Y., R. M. Hozalsk, L. W. Clapp, P. J. Novak, and M. J. Semmens (2002) Passive dissolution of hydrogen gas into groundwater using hollow-fiber membranes. Water Res. 36: 3533–3542.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Civil and Environmental System Engineering, Konkuk University, Seoul, 143-701, Korea

    Ki Young Park

  2. Department of Civil and Environmental Engineering, Sejong University, Seoul, 143-747, Korea

    Sung Kyu Maeng

  3. Center for Water Resources Cycle Research, Korea Institute of Science and Technology, Seoul, 136-791, Korea

    Kyu-Hong Ahn

  4. Department of Environmental Engineering, College of Science and Technology, Korea University, Sejong, 339-700, Korea

    Kwanhyoung Lee, Oh Kyung Choi & Jae Woo Lee

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  1. Ki Young Park
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  2. Sung Kyu Maeng
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  3. Kyu-Hong Ahn
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  4. Kwanhyoung Lee
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  5. Oh Kyung Choi
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  6. Jae Woo Lee
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Correspondence to Jae Woo Lee.

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Park, K.Y., Maeng, S.K., Ahn, KH. et al. Carbon dioxide injection method for enhancing hydrogenotrophic denitrification of secondary wastewater effluent in fixed bed reactor. Biotechnol Bioproc E 18, 326–332 (2013). https://doi.org/10.1007/s12257-012-0537-9

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  • DOI: https://doi.org/10.1007/s12257-012-0537-9

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