Abstract
Bio-oxidation of sulfide under denitrifying conditions is a key process in the treatment of gas and liquids that are contaminated with sulfide and nitrite. A lab-scale continuous flow stirred tank reactor (CFSTR) was operated with nitrite as the electron acceptor for the evaluation of the effects of loading rates, hydraulic retention time (HRT) and substrate concentrations on the performance of the autotrophic denitrification process. The influent sulfide concentration was maintained at 0.16 kg/m3 and the HRT was decreased from 8.4 to 2 h and for the entire study period, the sulfide removal efficiency was above 80% for the loading rates that ranged from 0.47 to 2.16 kg S−2/m3day. However, lower influent loading of NO2 −-N that correspond to the stoichiometric ratios was used and the nitrite removal efficiency was close to 100%. The stoichiometry of sulfide oxidation was calculated by assuming the different end-products based on thermodynamic approach. We compared the calculated values with experimental yield values.
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Janssen, A. J. H., H. Dijkman, and G. Janssen (2000) Novel biological processes for the removal of H2S and SO2 from gas streams. pp. 265–280. In: P. N. L. Lens and L. Hulshoff Pol (eds.) Environmental technologies to treat sulfur pollution: Principles and engineering. IWA Publisher, London, UK.
Beristain-Cardoso, R., R. Sierra-Alvarez, P. Rowlette, E. R. Flores, J. Gomez, and J. A. Field (2007) Sulphide oxidation under chemolithoautotrophic denitrifying conditions. Biotechnol. Bioeng. 95: 1148–1157.
Yavuz, B., M. Türker, and G. Ö. Engin (2007) Autotrophic removal of sulphide from industrial wastewaters using oxygen and nitrate as electron acceptors. Environ. Eng. Sci. 24: 457–470.
Can-Dogan, E., M. Türker, L. Dagasan, and A. Arslan (2010) Sulphide removal from industrial wastewaters by autotrophic denitrification using nitrate as an electron acceptor. Wat. Sci. Technol. 62: 2286–2293.
Perez R., A. Gali, J. Dosta, and J. Mata-Alvarez (2007) Biological nitrogen removal (BNR) using sulphides for autotrophic denitrification in a sequencing batch reactor (SBR) to treat reject water. Ind. Eng. Chem. Res. 46: 6646–6649.
Li, W., Q. -L. Zhao, and H. Liu (2009) Sulphide removal by simultaneous autotrophic and heterotrophic desulfurization-denitrification process. J. Hazard. Mater. 162: 848–853.
Moon, H. S., K. H. Ahn, S. Lee, K. Nam, and J. Y. Kim (2004) Use of autotrophic sulfur-oxidizers to remove nitrate from bank filtrate in a permeable reactive barrier system. Environ. Pollut. 129: 499–507.
Mahmood, Q., P. Zheng, J. Cai, D. Wu, B. Hu, and J. Li (2007) Anoxic sulphide biooxidation using nitrite as electron acceptor. J. Hazard. Mater. 147: 249–256.
Sierra-Alvarez, R., R. Beristain-Cardosa, M. Salazar, J. Gomez, E. Razo-Flores, and J. A. Field (2007) Chemolithotrophic denitrification with elementel sulfur for groundwater treatment. Wat. Res. 41: 1253–1262.
Campos, J. L., S. Carvalho, R. Portela, A. Mosquera-Corral, and R. Mendez (2008) Kinetics of denitrification using sulfur compounds: Effects of S/N ratio, endogenous and exogenous compounds. Biores. Technol. 99: 1293–1299.
Jing, C., Z. Ping, and Q. Mahmood (2008) Effect of sulfide to nitrate ratios on the simultaneous anaerobic sulphide and nitrate removal. Biores. Technol. 99: 5520–5527.
Jing, C., Z. Ping, and Q. Mahmood (2010) Influence of various nitrogenous electron acceptors on the anaerobic sulfide oxidation. Biores. Technol. 101: 2931–2937.
Manconi, I., P. Carucci Lens, and S. Rossetti (2006) Simultaneous biological removal of sulphide and nitrate by autotrophic denitrification in an activated sludge system. Wat. Sci. Technol. 53: 91–99.
Beristain-Cardoso, R., A. -C. Texier, R. Sierra-Alvarez, J. A. Field, E. Razo-Flores, and J. Gomez (2008) Simultaneous sulphide and acetate oxidation under denitrifying conditions using an inverse fludized bed reactor. J. Chem. Technol. Biotechnol. 83: 1197–1203.
Mahmood, Q., P. Zheng, Y. Hayat, E. Islam, D. Wu, and J. Ren- Cun (2008) Effect of pH on anoxic sulphide oxidizing reactor performance. Biores. Technol. 99: 3291–3296.
Başpınar, A. B., M. Turker, A. Hocalar, and I. Öztürk (2011) Biogas desulphurization at technical scale by lithotrophic denitrification: Integration of sulphide and nitrogen removal. Proc. Biochem. 46: 916–922.
Türker, M., A. B. Başpınar, and A. Hocalar (2012) Monitoring and control of biogas desulphurization using oxidation reduction potential under denitrifiying conditions. J. Chem. Tech. Biotech. 187: 682–688.
APHA, AWWA (2005) Standard Methods for the Examination of Water and Wastewaters. 21th ed., American Public Health Association Publication, Washington, USA.
Buisman, C. J. N., P. Ijspeert, A. Hof, A. J. H. Janssen, R. Hagen, and G. Lettýnga (1991) Kinetic parameters of mixed culture oxidizing sulfide and sulfur with oxygen. Biotechnol. Bioeng. 38: 813–820.
Nishimura, S. and M. Yoda (1997) Removal of hydrogen sulphide from an anaerobic biogas using a bio-scrubber. Wat. Sci. Technol. 36: 349–356.
Barbosa, V. L., J. E. Buergess, K. Dark, and R. M. Stuetz (2002) Activated sludge biotreatment of sulfurous waste emissions. Environ. Sci. Biotechnol. 1: 345–362.
Kleerebezem, R. and R. Mendez (2002) Autotrophic denitrification for combined hydrogen sulphide removal from biogas and post-denitrification. Wat. Sci. Technol. 45: 349–356.
Vaiopoulou, E., P. Melidis, and A. Aivasidis (2005) Sulphide removal in wastewater from petrochemical industries by autotrophic denitrification. Wat. Res. 39: 4101–4109.
Mahmood, Q., P. Zheng, J. Cai, D. Wu, B. Hu, E. Isiam, and M. R. Azim (2007) Comparision of anoxic sulfide biooxidation using nitrate/nitrite as electron acceptor. Environ. Prog. 26: 169–177.
Rittman, B. E. and P. L. Mc Carty (2001) Environmental biotechnology: Principles and applications. pp. 132–145. McGraw Hill Higher Education, America.
Tchobanoglous, G., F. L. Burton, and H. D. Stensel (2003) Wastewater Engineering, Treatment and Reuse. 4th ed. pp. 571–579. Metcalf & Eddy, Inc. Mc Graw-Hill Companies, NY, USA.
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Doğan, E.C., Türker, M., Dağaşan, L. et al. Simultaneous sulfide and nitrite removal from industrial wastewaters under denitrifying conditions. Biotechnol Bioproc E 17, 661–668 (2012). https://doi.org/10.1007/s12257-011-0677-3
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DOI: https://doi.org/10.1007/s12257-011-0677-3