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Comparative lipidomic analysis of Cephalosporium acremonium insights into industrial and pilot fermentations

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Abstract

Cephalosporium acremonium has been widely applied in industrial cephalosporin C fermentation. However, little is known about the molecular basis of fermentation behavior of this strain. In this study, comparative lipidomic analysis using LC/ESI/MSn technology was employed to investigate responses of Cephalosporium acremonium to multiple environment variations in realistic industrial cephalosporin C fermentation process and provide molecular basis for the discrepancies between industrial and pilot fermentations. Totally 77 phospholipids species were detected and 65 species were further quantified. Score plot revealed that phospholipids metabolism differed in industrial and pilot process. Loading pilot indicated that the main variables responsible for the discrimination of industrial and pilot process were phosphatidylinositols (PIs), phosphatidylserines (PSs) and phosphatic acids (PAs). Higher PIs content in industrial process indicated that cells were more vigorous in industrial process than those in pilot process. Larger increases of PSs, PAs and ratio of oleic acid to linoleic acid coincided well with the earlier and more thorough cellular morphological differentiation in industrial process. The synergetic reaction between cellular behavior and cells living environment led to titer discrepancies between industrial and pilot process. These findings provided lipidomic insights into industrial cephalosporin C production.

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Authors and Affiliations

  1. Key Laboratory of Systems Bioengineering of Ministry of Education, Department of pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300-072, China

    Rui-Juan Xu, Bin Qiao, Bing-Zhi Li & Ying-Jin Yuan

  2. CSPC Hebei Zhongrun Pharmaceutical Co., Ltd, Shijiazhuang, Hebei, 050-041, China

    Hua Lu & Yao Chen

Authors
  1. Rui-Juan Xu
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  2. Bin Qiao
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  3. Bing-Zhi Li
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  4. Hua Lu
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  5. Yao Chen
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  6. Ying-Jin Yuan
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Correspondence to Ying-Jin Yuan.

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Xu, RJ., Qiao, B., Li, BZ. et al. Comparative lipidomic analysis of Cephalosporium acremonium insights into industrial and pilot fermentations. Biotechnol Bioproc E 17, 259–269 (2012). https://doi.org/10.1007/s12257-011-0494-8

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  • DOI: https://doi.org/10.1007/s12257-011-0494-8

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