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Screening and characterization of an aerobic nitrifying-denitrifying bacterium from activated sludge

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Abstract

Taking advantage of the good biocompatibility and high efficiency of nitrogen removal with microbes, nitrifying and denitrifying bacteria, are becoming increasingly more widely used for wastewater treatment and prevention of eutrophication. In this research, an aerobic nitrifying-denitrifying bacterium was successfully screened from activated sludge and identified as Pseudomonas sp. (CCTCC No M2010209) by the 16S rDNA sequence. The activity verification confirmed its nitrifying-denitrifying capability of removing ammonium, nitrate and nitrite nitrogen. The types of carbon sources and carbon-nitrogen ratio greatly influenced the removal efficiency of NH4 +-N and NO3 −-N. When the initial concentrations of NH4 +-N and NO3 −-N in synthetic wastewater were less than 70 and 50 mg/L, the nitrogen removal rates reached 94 and 90% in 9 h, respectively. Preliminary comparisons of nitrogen removal capacity between this isolate and other commercial preparations in the treatment of synthetic wastewater revealed its promising potential to be used in the actual wastewater treatment.

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Authors and Affiliations

  1. Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214-122, China

    Xiaofan Qiu, Tianwen Wang, Xiaomin Zhong, Guocheng Du & Jian Chen

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  1. Xiaofan Qiu
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  2. Tianwen Wang
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  3. Xiaomin Zhong
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  4. Guocheng Du
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  5. Jian Chen
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Correspondence to Guocheng Du or Jian Chen.

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Qiu, X., Wang, T., Zhong, X. et al. Screening and characterization of an aerobic nitrifying-denitrifying bacterium from activated sludge. Biotechnol Bioproc E 17, 353–360 (2012). https://doi.org/10.1007/s12257-011-0467-y

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  • DOI: https://doi.org/10.1007/s12257-011-0467-y

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