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Immunostimulatory effect by aqueous extract of Hizikia fusiforme in RAW 264.7 macrophage and whole spleen cells

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Abstract

Hizikia fusiforme is a commonly used food that possesses potent anti-bacterial, anti-fungal, and anti-inflammatory activities. The immunostimulatory activities of aqueous extract of Hizikia fusiforme (HFAE) in RAW 264.7 macrophages and whole spleen cells were investigated. HFAE activated RAW 264.7 macrophages to produce cytokines such as nitric oxide (NO), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) in a dose-dependent manner. In addition, HFAE induced the mRNA expression of TNF-α, IL-1β, and IL-6 in RAW 264.7 macrophages. Moreover, HFAE stimulated proliferation of whole spleen cells and reference mitogen. Taken together, the results demonstrate that HFAE potently activates the immune function by regulating NO, TNF-α, IL-1β, and IL-6 in RAW 264.7 macrophage and promoting spleen cell proliferation.

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Authors and Affiliations

  1. Bioevaluation Center, Korea Research Institute of Bioscience and Biotechnology, Ochang, 363-887, Korea

    Yeo Dae Yoon

  2. Departments of Herbal Skin Care, Daegu Hanny University, Gyeongsan, 712-715, Korea

    Eun Sook Lee & Jin Hee Kim

  3. Herbal Pharmaceutical Engineering, Daegu Hanny University, Gyeongsan, 712-715, Korea

    Jong Pil Park

  4. Department of Food and Nutrition, Chungnam National University, Daejeon, 305-764, Korea

    Mee Ree Kim

  5. Department of Life Science and Genetic Engineering, Paichai University, Daejeon, 302-735, Korea

    Jun Won Lee

  6. College of Pharmacy, Yeungnam University, Gyeongsan, 712-749, Korea

    Tae Hoon Kim & Min Kyun Na

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  1. Yeo Dae Yoon
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  2. Eun Sook Lee
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Correspondence to Min Kyun Na or Jin Hee Kim.

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Yoon, Y.D., Lee, E.S., Park, J.P. et al. Immunostimulatory effect by aqueous extract of Hizikia fusiforme in RAW 264.7 macrophage and whole spleen cells. Biotechnol Bioproc E 16, 1099–1105 (2011). https://doi.org/10.1007/s12257-011-0177-5

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  • DOI: https://doi.org/10.1007/s12257-011-0177-5

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