Protective effect of GABA-enriched fermented sea tangle against ethanol-induced cytotoxicity in HepG2 Cells

  • Young-Mi Kang
  • Zhong-Ji Qian
  • Bae-Jin Lee
  • Young-Mog KimEmail author
Research Paper


This study was conducted to evaluate the protective effects of GABA (gamma-amino butyric acid)-enriched sea tangle juice (STJ) by Lactobacillus brevis BJ-20 fermentation against alcohol hepatotoxicity. The protective effects were determined by assessing glutathione (GSH) content levels and gamma-glutamyl transpeptidase (GGT) activity against ethanol-induced cytotoxicity in HepG2 cells. In ethanol-treated cells, GSH content decreased to 44.35% of control (ethanol-untreated cell) values; however, treatment with fermented sea tangle juice (FSTJ) at a concentration of 25 μg/mL increased GSH levels to 67.08%.These results suggest that FSTJ may prevent intracellular GSH depletion caused by ethanol consumption. Treatment with FSTJ against alcohol-injured HepG2 cells resulted in a dose-dependent decrease in GGT activity. The expression of cytochrome P450 2E1 (CYP2E1) enzyme, a major contributor to ethanol-induced oxidative stress, was also completely inhibited in FSTJ-treated cells at a concentration of 25 μg/mL. Thus, this study demonstrated that ethanolinduced cytotoxicity could be attenuated by inhibition of GSH depletion, GGT activity, and CYP2E1 expression.


alcohol hepatocytotoxicity gamma-glutamyl transpeptidase fermented sea tangle juice HepG2 cells 


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Copyright information

© The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg 2011

Authors and Affiliations

  • Young-Mi Kang
    • 1
  • Zhong-Ji Qian
    • 2
    • 3
  • Bae-Jin Lee
    • 1
  • Young-Mog Kim
    • 4
    Email author
  1. 1.Marine Bioprocess Co., Ltd.BusanKorea
  2. 2.Department of Marine Life Science and Marine Life Research & Education CenterChosun UniversityGwangjuKorea
  3. 3.Department of Marine Life Science and Marine Life Research & Education CenterChosun UniversityWandoKorea
  4. 4.Department of Food Science and TechnologyPukyong National UniversityBusanKorea

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