Skip to main content
SpringerLink
Log in

Immunostick assay for medical diagnosis of rheumatoid arthritis

  • Research Paper
  • Published:
Biotechnology and Bioprocess Engineering Aims and scope Submit manuscript

Abstract

An immunoassay based on stick-type solid support (immunostick assay) was developed. To demonstrate the medical diagnosis of rheumatoid arthritis (RA), cyclic-citrullinated peptide (CCP), one of specific antigens against RA autoantibodies, was immobilized on the surface of the immunostick, and a color index table was prepared for the determination of CCP-positivity of the test sera. The positivity of RA-positive (n = 31) and RA-negative (n = 20) sera was tested using the immunostick assay and a conventional enzyme-linked immunosorbent assay (ELISA). The statistical agreement of the test results from both methods was analyzed using inter-rater coefficient kappa and Bland-Altman test. The immunostick assay with a color index table was determined to have a high statistical correlation to the conventional ELISA method.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Ekins, R. P. (1999) Immunoassay and other ligand assays: From isotopes to luminescence. J. Clin. Ligand Assay 22: 61–77.

    Google Scholar 

  2. Gosling, J. P. (2000) Immunoassays. Oxford University Press, Oxford, UK.

    Google Scholar 

  3. Asensio, L., I. González, T. García, and R. Martín (2008) Determination of food authenticity by enzyme-linked immunosorbent assay (ELISA). Food Control. 19: 1–8.

    Article  CAS  Google Scholar 

  4. Hurley, I. P., R. Cook, C. W. Laughton, N. A. Pickles, H. E. Ireland, and J. H. H. Williams (2009) Detection of human blood by immunoassay for applications in forensic analysis. Forensic Sci. International. 190: 91–97.

    Article  CAS  Google Scholar 

  5. Wolfe, A. M., J. H. Kellgren, and A. T. Masi (1968) The epidemiology of rheumatoid arthritis: A review. II. Incidence and diagnostic criteria. Bull Rheum. Dis. 19: 524–529.

    CAS  Google Scholar 

  6. Visser, H., L. B. S. Gelinck, A. H. Kampfraath, C. Breedveld, and J. M. W. Hazes (1996) Diagnostic and prognostic characteristics of the enzyme linked immunosorbent rheumatoid factor assay in rheumatoid arthritis. Ann. Rheum. Dis. 55: 157–161.

    Article  CAS  Google Scholar 

  7. Harris, E. (1993) Etiology and pathogenesis of rheumatoid arthritis. pp. 833–868. In: W. Kelley, E. Harris, S. Ruddy, and C. Sledge (eds.). Kelley’s Textbook of Rheumatology. W. B. Saunders Company, Philadelphia, PA, USA.

    Google Scholar 

  8. Harris, E. (1993) Clinical features of rheumatoid arthritis. pp. 874–907. In: W. Kelley, E. Harris, S. Ruddy, and C. Sledge (eds.). Kelley’s Textbook of Rheumatology. W. B. Saunders Company, Philadelphia, PA, USA.

    Google Scholar 

  9. Schellekens, G. A., H. Visser, B. A. de Jong, F. H. van den Hoogen, J. M. Hazes, F. C. Breedveld, and W. J. van Venrooij (2000) The diagnostic properties of rheumatoid arthritis antibodies recognizing a cyclic citrullinated peptide. Arthritis Rheum. 43: 155–163.

    Article  CAS  Google Scholar 

  10. Nakamura, R. M. (2000) Progress in the use of biochemical and biological markers for evaluation of rheumatoid arthritis. J. Clin. Lab. Anal. 14: 305–313.

    Article  CAS  Google Scholar 

  11. Sebbag, M., M. Simon, C. Vincent, C. Masson-Bessiere, E. Girbal, J. J. Durieux, and G. Serre (1995) The antiperinuclear factor and the so-called antikeratin antibodies are the same rheumatoid arthritis-specific autoantibodies. J. Clin. Invest. 95: 2672–2679.

    Article  CAS  Google Scholar 

  12. Arnett, F. C., S. M. Edworthy, and D. A. Bloch (1988) The American Rheumatism Association 1987 revised criteria for the classification of rheumatoid arthritis. Arthritis Rheum. 31: 315–324.

    Article  CAS  Google Scholar 

  13. Jose, J., M. Park, and J. C. Pyun (2010) Highly sensitive immunoassay based on E. coli with autodisplayed Z-domain. Analytica Chim. Acta. 667: 113–118.

    CAS  Google Scholar 

  14. Mackinnon, A. (2000) A spreadsheet for the calculation of comprehensive statistics for the assessment of diagnostic tests and inter-rater agreement. Computers in Biol. Med. 30: 127–134.

    Article  CAS  Google Scholar 

  15. Bland, J. M. and D. G. Altman (1986) Statistical method for assessing agreement between two methods of clinical measurement. The Lancet i: 307–310.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Materials Science & Engineering, Yonsei University, Seoul, 120-749, Korea

    Hyuk Ko, Eun-Hang Lee, Byoung-Jin Jeon, Hye-Jin Choi & Jae-Chul Pyun

  2. Korea Institute of Science and Technology (KIST), Seoul, 136-130, Korea

    Min-Jung Kang

Authors
  1. Hyuk Ko
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Eun-Hang Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Byoung-Jin Jeon
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hye-Jin Choi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Min-Jung Kang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jae-Chul Pyun
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jae-Chul Pyun.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ko, H., Lee, EH., Jeon, BJ. et al. Immunostick assay for medical diagnosis of rheumatoid arthritis. Biotechnol Bioproc E 16, 1248–1253 (2011). https://doi.org/10.1007/s12257-011-0106-7

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12257-011-0106-7

Keywords

Search

Navigation