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Decolorization method of crude alkaline protease preparation produced from an alkalophilic Bacillus clausii

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Abstract

Diaion HPA75 decolorized efficiently the crude preparation of novel alkaline protease produced by Bacillus clausii. The optimum concentrations of HPA75 and contact time for efficient decolorization were determined to be approximately 6 ∼ 10% (w/v) and 8 h, respectively. Color removal efficiency was improved at alkaline pH, and 21% color intensity was retained with a protease yield of 99.7% at pH 11. By using highly concentrated samples, a pattern of decolorization was achieved that was similar to that produced by unconcentrated enzyme preparations. After treatment with 6% HPA75 for 8 h, the residual color intensity was approximately 20% with a protease yield of nearly 100%. Used HPA75 could be regenerated easily, and the regenerated HPA75 was as effective as the fresh HPA75 for decolorization and protease recovery. The regeneration efficiency of the used Diaion HPA75 was greater than 90% until it was used four times. Considering these results, we suggest Diaion HPA75 is suitable for color removal applications, producing high protease yields from fermented broth.

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Authors and Affiliations

  1. Department of Bioindustry, Daegu University, Kyungsan, 712-714, Korea

    Kwang Bon Koo & Jang Won Choi

  2. C & J Biotech, 4318 RIC, Hallym University, Chuncheon, 200-702, Korea

    Han-Seung Joo

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  1. Kwang Bon Koo
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Correspondence to Jang Won Choi.

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Koo, K.B., Joo, HS. & Choi, J.W. Decolorization method of crude alkaline protease preparation produced from an alkalophilic Bacillus clausii . Biotechnol Bioproc E 16, 89–96 (2011). https://doi.org/10.1007/s12257-010-0304-8

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  • DOI: https://doi.org/10.1007/s12257-010-0304-8

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