Abstract
Transesterification is a principal chemical reaction that occurs in biodiesel production. We developed a novel biocatalytic membrane microreactor (BMM) for continuous transesterification by utilizing an asymmetric membrane as an enzyme-carrier for immobilization. The BMM was developed by pressure driven filtration of lipase from Pseudomonas fluorescens, which is suitable for highly efficient biocatalytic transesterification. Lipase solution was allowed to permeate through an asymmetric membrane with NMWL 300 kDa composed of polyethersulfone. The performances of BMM were studied in biodiesel synthesis via transesterification of triolein with methanol. Transesterification was carried out by passing a solution of triolein and methanol through the asymmetric membrane. The degree of triolein conversion using this microreactor was ca. 80% with a reaction time of 19 min. The BMM system displayed good stability, with no activity decay over a period of 12 day with continuous operation. Results from triolein transesterification clearly demonstrate the potential of an asymmetric membrane as an enzyme carrier material. Enzyme activity (mmol/h·glipase) was approximately 3 fold higher than that of native free lipase.
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Zhao, X. S., X. Y. Bao, W. Guo, and F. Y. Lee (2006) Immobilizing catalysts on porous materials. Materials Today 9: 32–39.
Alonso, F. O. M., E. B. L. Oliveira, G. M. Dellamora-Ortiz, and F. V. Pereira-Meirelles (2005) Improvement of lipase production at different stirring speeds and oxygen levels. Braz. J. Chem. Eng. 22: 9–18.
Bornscheuer, U. T. (2003) Immobilizing enzymes: How to create more suitable biocatalysts. Angew. Chem. Int. Ed. 42: 3336–3337.
Villeneuve, P., J. M. Muderhwa, J. Graille, and M. J. Haas (2000) Customizing lipases for biocatalysis: A survey of chemical, physical, and molecular biological approaches: A review. J. Mol. Catal. B: Enzym. 9: 113–148.
Malcata, F. X., H. R. Reyes, H. S. Garcia, C. G. Hill, and C. H. Amundson (1990) Immobilized lipase reactors for modification of fats and oils: A review. J. Am. Oil Chem. Soc. 67: 890–910.
Mateo, C., J. M. Palomo, G. F. Lorente, J. M. Guisan, and R. F. Lafuente (2007) Improvement of enzyme activity, stability and selectivity via immobilization techniques. Enzym. Microb. Technol. 40: 1451–1463.
Luthfi, A., S. Setyahadi, M. Gozan, and M. Nasikin (2009) Continuous transesterification by biocatalytic membrane microreactor for synthesis of methyl ester. Proceeding of the 11 th International Conference on QiR (Quality in Research). August 3-6. Depok, Indonesia.
Rios, G. M., M. P. Belleville, D. Paolucci, and J. Sanchez (2004) Progress in enzymatic membrane reactors: A Review. J. Memb. Sci. 242: 189–196.
Balcao, V. M., A. L. Paiva, and F. X. Malcata (1996) Bioreactors with immobilized lipases: State of the art. Enz. Microb. Technol. 18: 392–416.
Vicente, G., M. Martinez, and J. Aracil (2004) Integrated biodiesel production: A comparison of different homogeneous catalysts systems. Bioresour. Technol. 92: 297–305.
Watanabe, Y., Y. Shimada, A. Sugihara, and Y. Tominaga (2001) Enzymatic conversion of waste edible oil to biodiesel fuel in a fixed-bed bioreactor. J. Am. Oil Chem. Soc. 78: 703–707.
Chen, J. W. and W. T. Wu (2003) Regeneration of immobilized Candida antarctica lipase for transesterification. J. Biosci. Bioeng. 95: 466–469.
Kim, J., J. W. Grate, and P. Wang (2006) Nanostructures for enzyme stabilization. Chem. Eng. Sci. 61: 1017–1026.
Holcapek, M., P. Jandera, J. Fisher, and B. Prokes (1999) Analytical monitoring of the production of biodiesel by high-performance liquid chromatography with various detection methods. J. Chromatogr. A. 858: 13–31.
Takahashi, H., B. Li, T. Sasaki, C. Miyazaki, T. Kajino, and Shinji Inagaki (2000) Catalytic activity in organic solvents and stability of immobilized enzymes depend on the pore size and surface characteristics of mesoporous silica. Chem. Mater. 12: 3301–3305.
Aimar, P., M. Meireles, P. Bacchin, and V. Sanches (1994) Fouling and concentration polarization in ultrafiltration and microfiltration. pp. 27–57. Volume 272. In: J. G. Crespo and K. W. Böddeker (eds.). Membrane Process in Separation and Purification; Kluwer Academic Publisher: Dordrecht, The Netherland.
Giorno, L., E. Drioli, G. Carvoli, A. Cassano, and L. Donato (2001) Study of an enzyme membrane reactor with immobilized fumarase for production of L-Malic acid. Biotech. Bioeng. 72: 77–84.
Hsu, A., K. C. Jones, T. A. Foglia, and W. N. Marmer (2004) Continuous production of ethyl esters of grease using an immobilized lipase. J. Am. Oil Chem. Soc. 81: 749–752.
Dossat, V., D. Combes, and A. Marty (1999) Continuous enzymatic transesterification of high oleic sunflower oil in a packed bed reactor: Influence of the glycerol production. Enzym. Microb. Technol. 25: 194–200.
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Machsun, A.L., Gozan, M., Nasikin, M. et al. Membrane microreactor in biocatalytic transesterification of triolein for biodiesel production. Biotechnol Bioproc E 15, 911–916 (2010). https://doi.org/10.1007/s12257-010-0151-7
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DOI: https://doi.org/10.1007/s12257-010-0151-7