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Improvement of tautomycin production in Streptomyces spiroverticillatus by feeding glucose and maleic anhydride

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Abstract

Optimization of the feeding process for tautomycin production by Streptomyces spiroverticillatus was performed using glucose and/or maleic anhydride. The feeding of glucose was based on the reducing sugar content (lower than 8 g/L) at a cultivation time of 40 h. After addition of 2% (w/v) glucose, the biomass increased from 21 to 28 g/L, and that of tautomycin from 572.06 to 837.6 mg/L. Moreover, 1723.1 mg/L of tautomycin (increased by 201.21%) was obtained by feeding 0.2% (w/v) maleic anhydride solution at a pH between 4 and 7 in the broth. For the experiments in the 15 L fermentor, tautomycin content reached its highest level (1714.7 mg/L), which was 199.7% higher than that of control by feeding both glucose and maleic anhydride.

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Authors and Affiliations

  1. Institute of Fermentation Engineering, College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, 310-032, China

    Xiao-Long Chen, Yu-Hua Xu, Yu-Guo Zheng & Yin-Chu Shen

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  1. Xiao-Long Chen
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  2. Yu-Hua Xu
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  3. Yu-Guo Zheng
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  4. Yin-Chu Shen
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Correspondence to Xiao-Long Chen.

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Chen, XL., Xu, YH., Zheng, YG. et al. Improvement of tautomycin production in Streptomyces spiroverticillatus by feeding glucose and maleic anhydride. Biotechnol Bioproc E 15, 969–974 (2010). https://doi.org/10.1007/s12257-010-0087-y

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  • DOI: https://doi.org/10.1007/s12257-010-0087-y

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