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Optimization of a liquid medium for beauvericin production in Fusarium redolens Dzf2 mycelial culture

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Abstract

Beauvericin (BEA) is a proven and potent antibiotic compound useful for bio-control and a potential antifungal and anticancer agent for human. This study was to evaluate and optimize the nutrient medium for BEA production in mycelial liquid culture of a high BEA-producing fungus Fusarium redolens Dzf2 isolated from a medicinal plant. Among various organic and inorganic carbon and nitrogen sources, glucose and peptone were found the most favorable for the F. redolens Dzf2 mycelial growth and BEA production. Through a Plackett-Burman screening test on a basal medium, glucose, peptone, and medium pH were identified as the significant factors for mycelial growth and BEA production. These factors were optimized through central composite design of experiments and response surface methodology, as 49.0 g/L glucose, 13.0 g/L peptone and pH 6.6, yielding 198 mg/L BEA (versus 156 mg/L in the basal medium). The BEA yield was further increased to 234 mg/L by feeding 10 g/L glucose to the culture during exponential phase. The results show that F. redolens Dzf2 mycelial fermentation is a feasible and promising process for production of BEA.

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Authors and Affiliations

  1. Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100094, China

    Li-Jian Xu & Li-Gang Zhou

  2. Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, China

    Li-Jian Xu, Yuan-Shuai Liu & Jian-Yong Wu

  3. College of Agricultural Resource and Environment, Heilongjiang University, Harbin, 150000, China

    Li-Jian Xu

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  1. Li-Jian Xu
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  2. Yuan-Shuai Liu
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Correspondence to Li-Gang Zhou or Jian-Yong Wu.

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Xu, LJ., Liu, YS., Zhou, LG. et al. Optimization of a liquid medium for beauvericin production in Fusarium redolens Dzf2 mycelial culture. Biotechnol Bioproc E 15, 460–466 (2010). https://doi.org/10.1007/s12257-009-3031-2

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  • DOI: https://doi.org/10.1007/s12257-009-3031-2

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