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High yield Rhizopus chinenisis prolipase production in Pichia pastoris: Impact of methanol concentration

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Abstract

Rhizopus lipases have been successfully expressed in Pichia pastors and different fermentation strategies have been investigated. However, there is no sufficient study on the effects of methanol concentration on the production of Rhizopus lipases in P. pastors. In this study, the lipase from Rhizopus chinensis CCTCC M20102 was expressed under different fed-batch fermentation conditions at methanol concentrations ranging from 0.5 to 3.5 g/L. The lipase activity, stability, and productivities were analyzed. The optimum methanol concentration was 1 g/L, with the highest lipase activity of 2,130 U/mL, without degradation. Additional information was obtained from the analysis of methanol consumption and production rates. The results also suggested that the cell concentration at the end of the glycerol fed-batch phase was very important for cell viability and protease activity.

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Authors and Affiliations

  1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214-122, China

    Dan Wu

  2. Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214-122, China

    Xiao Wei Yu, Tong Chun Wang, Rui Wang & Yan Xu

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  1. Dan Wu
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  2. Xiao Wei Yu
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  3. Tong Chun Wang
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  4. Rui Wang
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  5. Yan Xu
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Correspondence to Xiao Wei Yu.

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Wu, D., Yu, X.W., Wang, T.C. et al. High yield Rhizopus chinenisis prolipase production in Pichia pastoris: Impact of methanol concentration. Biotechnol Bioproc E 16, 305–311 (2011). https://doi.org/10.1007/s12257-009-3021-4

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  • DOI: https://doi.org/10.1007/s12257-009-3021-4

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