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Methacryloylamidohistidine in affinity ligands for immobilized metal-ion affinity chromatography of ferritin

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Abstract

A new metal-chelate adsorbent utilizing 2-methacryloylamidohistidine (MAH) was prepared as a metalchelating ligand. MAH was synthesized using methacryloly chloride and histidine. Monosize nanospheres with an average diameter of 450 nm were produced by emulsion polymerization of 2-hydroxyetylmethacrylate (HEMA) and MAH. Then, Fe3+ ions were chelated directly onto the monosize nanospheres. Mon-poly(HEMA-MAH) nanospheres were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, and elemental analysis. Fe3+ chelated monosize nanospheres were used in ferritin adsorption from an aqueous solution. The maximum ferritin adsorption capacity of Fe3+-chelated mon-poly(HEMAMAH) nanospheres was 202 mg/g at pH 4.0 in acetate buffer. The non-specific ferritin adsorption on the monpoly( HEMA-MAH) nanospheres was 20 mg/g. The adsorption behavior of ferritin could be modeled using both Langmuir and Freundlich isotherms. The adsorption capacity decreased with increasing ionic strength of the binding buffer. High desorption ratios (> 95% of the adsorbed ferritin) were achieved with 1.0 M NaCl at pH 7.0. Ferritin could be repeatedly adsorbed and desorbed with the Fe3+-chelated mon-poly(HEMA-MAH) nanospheres without significant loss of adsorption capacity.

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Authors and Affiliations

  1. Adnan Menderes University Faculty of Science and Arts Chemistry Department, Aydin, 09010, Turkey

    Deniz Aktaş Uygun & Nevra Öztürk

  2. Faculty of Science, Biochemistry Department, Ege University, Izmir, 35100, Turkey

    Sinan Akgöl

  3. Faculty of Science, Chemistry Department, Hacettepe University, Ankara, 06532, Turkey

    Adil Denizli

Authors
  1. Deniz Aktaş Uygun
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  2. Nevra Öztürk
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  3. Sinan Akgöl
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  4. Adil Denizli
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Correspondence to Sinan Akgöl.

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Uygun, D.A., Öztürk, N., Akgöl, S. et al. Methacryloylamidohistidine in affinity ligands for immobilized metal-ion affinity chromatography of ferritin. Biotechnol Bioproc E 16, 173–179 (2011). https://doi.org/10.1007/s12257-009-0162-4

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  • DOI: https://doi.org/10.1007/s12257-009-0162-4

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