Biotechnology and Bioprocess Engineering

, Volume 15, Issue 1, pp 167–172 | Cite as

Widdrol from Juniperus chinensis induces apoptosis in human colon adenocarcinoma HT29 Cells

  • Hyun-Ju Kwon
  • Eun-Woo Lee
  • Yun-Kyung Hong
  • Hee-Jung Yun
  • Byung-Woo KimEmail author


Juniperus chinensis is a well-known folk remedy in Korea, shown to exhibit anti-tumor, anti-bacterial, anti-fungal, abortifacient, anti-platelet, vasorelaxing, and anti-viral activities. In this study, we report the cytotoxic activity as well as the apoptotic mechanism of widdrol extracted from Juniperus chinensis against the human colon adenocarcinoma cell line HT29. Treatment of HT29 cells with various concentrations of widdrol inhibited growth and induced apoptosis in a dose-dependent manner, as determined by cell viability, chromatin condensation, and sub-G1 phase accumulation. Western blot analysis revealed that apoptosis of HT29 cells was associated with release of cytochrome C from mitochondria into the cytosol, activation of caspases-3, -8, and -9, and proteolytic cleavage of PARP. These results suggest that widdrol exhibits an anti-proliferative effect on HT29 cells via apoptosis and can be a potential candidate in the field of anti-cancer drug discovery.


plant widdrol Juniperus chinensis apoptosis HT29 cell anti-cancer anti-proliferative activity 


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Copyright information

© The Korean Society for Biotechnology and Bioengineering and Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  • Hyun-Ju Kwon
    • 1
    • 2
    • 3
  • Eun-Woo Lee
    • 1
    • 3
  • Yun-Kyung Hong
    • 1
  • Hee-Jung Yun
    • 2
  • Byung-Woo Kim
    • 1
    • 2
    • 3
    Email author
  1. 1.Department of Life Science and Biotechnology, College of Natural ScienceDongeui UniversityBusanKorea
  2. 2.Department of Biomaterial ControlDongeui University Graduate SchoolBusanKorea
  3. 3.Blue-Bio Industry RICDongeui UniversityBusanKorea

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