Skip to main content
SpringerLink
Log in

Effects of low temperature and lactate on osteogenic differentiation of human amniotic mesenchymal stem cells

  • Articles
  • Published:
Biotechnology and Bioprocess Engineering Aims and scope Submit manuscript

Abstract

A functional relationship between the growth and the progression of events associated with osteogenic differentiation of human amniotic mesenchymal stem cells (hAMSCs) has been a fundamental question, which remains unclear. This study is aimed at investigating the effects of low temperature and lactate individually, and in combination on the growth and osteogenic differentiation of hAMSCs. It was shown that the growth of hAMSCs in growth medium was inhibited by both low-cultivation temperature and lactate. By extending culture period at low temperature, cell growth declined gradually, while the ALP expression and calcium deposition increased progressively. However, the growth of hAMSCs induced in osteogenic medium at 37°C was markedly enhanced by additional lactate. The ALP expression and calcium deposition, on the contrary, were significantly depressed. Furthermore, the synergistic actions of long-term low temperature and lactate resulted in more intense inhibition on both cell growth and osteogenic differentiation. Therefore, these findings may imply the co-contribution of the culture environment on the selective manipulation on the growth capacity and osteogenic differentiation potential of hAMSCs.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Kon, E., A. Muraglia, A. Corsi, P. Bianco, M. Marcacci, I. Martin, A. Boyde, I. Ruspantini, P. Chistolini, M. Rocca, R. Giardino, R. Cancedda, and R. Quarto (2000) Autologous bone marrow stromal cells loaded onto porous hydroxyapatite ceramic accelerate bone repair in critical-size defects of sheep long bones. J. Biomed. Mater. Res. 49: 328–337.

    Article  CAS  Google Scholar 

  2. Petite, H., V. Viateau, W. Bensaid, A. Meunier, C. de Pollak, M. Bourguignon, K. Oudina, L. Sedel, and G. Guillemin (2000) Tissue-engineered bone regeneration. Nat. Biotechnol. 18: 959–963.

    Article  CAS  Google Scholar 

  3. Barlow, S., G. Brooke, K. Chatterjee, G. Price, R. Pelekanos, T. Rossetti, M. Doody, D. Venter, S. Pain, K. Gilshenan, and K. Atkinson (2008) Comparison of human placenta- and bone marrow-derived multipotent mesenchymal stem cells. Stem Cells Dev. 17: 1095–1108.

    Article  Google Scholar 

  4. Brooke, G., H. Tong, J. P. Levesque, and K. Atkinson (2008) Molecular trafficking mechanisms of multipotent mesenchymal stem cells derived from human bone marrow and placenta. Stem Cells Dev. 17: 929–940.

    Article  CAS  Google Scholar 

  5. Bonab, M. M., K. Alimoghaddam, F. Talebian, S. H. Ghaffari, A. Ghavamzadeh, and B. Nikbin (2006) Aging of mesenchymal stem cell in vitro. BMC Cell Biol. 7: 14.

    Article  CAS  Google Scholar 

  6. Kretlow, J. D., Y. Q. Jin, W. Liu, W. J. Zhang, T. H. Hong, G. D. Zhou, L. S. Baggett, A. G. Mikos, and Y. L. Cao (2008) Donor age and cell passage affects differentiation potential of murine bone marrow-derived stem cells. BMC Cell Biol. 9: 60.

    Article  CAS  Google Scholar 

  7. Choi, K. M., Y. K. Seo, H. H. Yoon, K. Y. Song, S. Y. Kwon, H. S. Lee, and J. K. Park (2007) Effects of mechanical stimulation on the proliferation of bone marrow-derived human mesenchymal stem cells. Biotechnol. Bioprocess Eng. 12: 601–609.

    Article  CAS  Google Scholar 

  8. Balint, E., P. Szabo, C. F. Marshall, and S. M. Sprague (2001) Glucose-induced inhibition of in vitro bone mineralization. Bone 28: 21–28.

    Article  CAS  Google Scholar 

  9. Rodriguez, J. P., S. Rios, and M. Gonzalez (2002) Modulation of the proliferation and differentiation of human mesenchymal stem cells by copper. J. Cell Biochem. 85: 92–100.

    Article  CAS  Google Scholar 

  10. Stein, G. S. and J. B. Lian (1993) Molecular mechanisms mediating proliferation/differentiation interrelationships during progressive development of the osteoblast phenotype. Endocr. Rev. 14: 424–442.

    CAS  Google Scholar 

  11. Kim, G. S., J. S. Hong, S. W. Kim, J. M. Koh, C. S. An, J. Y. Choi, and S. L. Cheng (2003) Leptin induces apoptosis via ERK/cPLA2/cytochrome c pathway in human bone marrow stromal cells. J. Biol. Chem. 278: 21920–21929.

    Article  CAS  Google Scholar 

  12. Doucet, C., I. Ernou, Y. Zhang, J. R. Llense, L. Begot, X. Holy, and J. J. Lataillade (2005) Platelet lysates promote mesenchymal stem cell expansion: a safety substitute for animal serum in cell-based therapy applications. J. Cell Physiol. 205: 228–236.

    Article  CAS  Google Scholar 

  13. Qu, W. J., D. B. Zhong, P. F. Wu, J. F. Wang, and B. Han (2008) Sodium fluoride modulates caprine osteoblast proliferation and differentiation. J. Bone Miner. Metab. 26: 328–334.

    Article  CAS  Google Scholar 

  14. Nagarathnamma, M. M. Rajan, and G. K. Sureshkumar (1997) Apoptosis extent increases in hybridoma cultures with temperature. Biotechnol. Lett. 19: 669–673.

    Article  CAS  Google Scholar 

  15. Stolzing, A., S. Sethe, and A. M. Scutt (2006) Stressed stem cells: temperature response in aged mesenchymal stem cells. Stem Cells Dev. 15: 478–487.

    Article  CAS  Google Scholar 

  16. Yoon, S. K., J. Y. Song, and G. M. Lee (2003) Effect of low culture temperature on specific productivity, transcription level, and heterogeneity of erythropoietin in Chinese hamster ovary cells. Biotechnol. Bioeng. 82: 289–298.

    Article  CAS  Google Scholar 

  17. Shui, C. and A. Scutt (2001) Mild heat shock induces proliferation, alkaline phosphatase activity, and mineralization in human bone marrow stromal cells and Mg-63 cells in vitro. J. Bone Miner. Res. 16: 731–741.

    Article  CAS  Google Scholar 

  18. Stolzing, A. and A. Scutt (2006) Effect of reduced culture temperature on antioxidant defences of mesenchymal stem cells. Free Radic. Biol. Med. 41: 326–338.

    Article  CAS  Google Scholar 

  19. Ozturk, S. S., M. R. Riley, and B. O. Palsson (1992) Effect of ammonia and lactate on hybridoma growth, metabolism, and antibody production. Biotechnol. Bioeng. 39: 418–431.

    Article  CAS  Google Scholar 

  20. Follmar, K. E., F. C. Decroos, H. L. Prichard, H. T. Wang, D. Erdmann, and K. C. Olbrich (2006) Effect of glutamine, glucose, and oxygen concentration on the metabolism and proliferation of rabbit adipose-derived stem cells. Tissue Eng. 12: 3525–3533.

    Article  CAS  Google Scholar 

  21. Grayson, W. L., F. Zhao, R. Izadpanah, B. Bunnell, and T. Ma (2006) Effects of hypoxia on human mesenchymal stem cell expansion and plasticity in 3D constructs. J. Cell Physiol. 207: 331–339.

    Article  CAS  Google Scholar 

  22. Wang, D. W., B. Fermor, J. M. Gimble, H. A. Awad, and F. Guilak (2005) Influence of oxygen on the proliferation and metabolism of adipose derived adult stem cells. J. Cell Physiol. 204: 184–191.

    Article  CAS  Google Scholar 

  23. Nicoll S. B., A. Wedrychowska, N. R. Smith, and R. S. Bhatnagar (2001) Modulation of proteoglycan and collagen profiles in human dermal fibroblasts by high density micromass culture and treatment with lactic acid suggests change to a chondrogenic phenotype. Connect. Tissue Res. 42: 59–69.

    Article  CAS  Google Scholar 

  24. Choi, Y. S., D. Y. Lee, I. Y. Kim, H. J. Kim, H. W. Park, T. B. Choe, and I. H. Kim (2007) Enhancement of erythropoietin production in recombinant Chinese hamster ovary cells by sodium lactate addition. Biotechnol. Bioprocess Eng. 12: 60–72.

    Article  CAS  Google Scholar 

  25. Patel, S. D., E. T. Papoutsakis, J. N. Winter, and W. M. Miller (2000) The lactate issue revisited: novel feeding protocols to examine inhibition of cell proliferation and glucose metabolism in hematopoietic cell cultures. Biotechnol. Prog. 16: 885–892.

    Article  CAS  Google Scholar 

  26. Chen, T., Y. Zhou, and W. S. Tan (2009) Influence of lactic acid on the proliferation, metabolism, and differentiation of rabbit mesenchymal stem cells. Cell Biol. Toxicol. 25: 573–586.

    Article  CAS  Google Scholar 

  27. Fedde, K. N., L. Blair, J. Silverstein, S. P. Coburn, L. M. Ryan, R. S. Weinstein, K. Waymire, S. Narisawa, J. L. Millan, G. R. Macgregor, and M. P. Whyte (1999) Alkaline phosphatase knock-out mice recapitulate the metabolic and skeletal defects of infantile hypophosphatasia. J. Bone Miner. Res. 14: 2015–2026.

    Article  CAS  Google Scholar 

  28. Ludwig, A., J. Tomeczkowski, and G. Kretzmer (1992) Influence of the temperature on the shear stress sensitivity of adherent BHK 21 cells. Appl. Microbiol. Biotechnol. 38: 323–327.

    Article  CAS  Google Scholar 

  29. Ryoo, H. M., A. J. van Wijnen, J. L. Stein, J. B. Lian, and G. S. Stein (1997) Detection of a proliferation specific gene during development of the osteoblast phenol-type by mRNA differential display. J. Cell Biochem. 64: 106–116.

    Article  CAS  Google Scholar 

  30. Siddhanti, S. R. and L. D. Quarles (1994) Molecular to pharmacologic control of osteoblast proliferation and differentiation. J. Cell Biochem. 55: 310–320.

    Article  CAS  Google Scholar 

  31. Bellows, C. G., J. N. Heersche, and J. E. Aubin (1999) Aluminum accelerates osteoblastic differentiation but is cytotoxic in long-term rat calvaria cell cultures. Calcif. Tissue Int. 65: 59–65.

    Article  CAS  Google Scholar 

  32. Cruz, H. J., C. M. Freitas, P. M. Alves, J. L. Moreira, and M. J. T. Carrondo (2000) Effects of ammonia and lactate on growth, metabolism, and productivity of BHK cells. Enzym. Microb. Technol. 27: 43–52.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200-237, China

    Ting Chen, Yan Zhou & Wen-Song Tan

Authors
  1. Ting Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yan Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Wen-Song Tan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Wen-Song Tan.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chen, T., Zhou, Y. & Tan, WS. Effects of low temperature and lactate on osteogenic differentiation of human amniotic mesenchymal stem cells. Biotechnol Bioproc E 14, 708–715 (2009). https://doi.org/10.1007/s12257-009-0034-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12257-009-0034-y

Keywords

Search

Navigation