Abstract
This study examined the effectiveness of ammonia percolation pretreatment of wheat straw for ethanol production. Ground wheat straw at a 10% (w/v) loading was pretreated with a 15% (v/v) ammonia solution. The experiments were performed at treatment temperature of 50∼170°C and residence time of 10∼150 min. The solids treated with the ammonia solution showed high lignin degradation and sugar availability. The pretreated wheat straw was hydrolyzed by a cellulase complex (NS50013) and β-glucosidase (NS50010) at 45°C. After saccharification, Saccharomyces cerevisiae was added for fermentation. The incubator was rotated at 120 rpm at 35°C. As a result of the pretreatment, the delignification efficiency was > 70% (170°C, 30 min) and temperature was found to be a significant factor in the removal of lignin than the reaction time. In addition, the saccharification results showed an enzymatic digestibility of > 90% when 40 FPU/g cellulose was used. The ethanol concentration reached 24.15 g/L in 24 h. This paper reports a total process for bioethanol production from agricultural biomass and an efficient pretreatment of lignocellulosic material.
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References
McKendry, P. (2002) Energy production from biomass (part 1): overview of biomass. Bioresour. Technol. 83: 37–46.
Ingram, L. O. and J. Doran (1995) Conversion of cellulosic materials to ethanol. FEMS Microbiol. Rev. 16: 235–241.
Schell, D. J., J. Farmer, M. Newman, and J. D. McMillan (2003) Dilute sulfuric acid pretreatment of corn stover in pilot-scale reactor investigation of yields, kinetics, and enzymatic digestibilities of solids. Appl. Biochem. Biotechnol. 105: 69–85.
Chang, V. and M. Holtzapple (2000) Fundamental factors affecting biomass enzymatic reactivity. Appl. Biochem. Biotechnol. 84–86: 5–37.
Chen, L. J., Y. L. Xu, F. W. Bai, W. A. Anderson, and M. Y. Murray (2005) Observed quasi-steady kinetics of yeast cell growth and ethanol formation under very high gravity fermentation condition. Biotechnol. Bioprocess Eng. 10: 115–121.
Moiser, N., C. Wyman, B. Dale, R. Elander, Y. Y. Lee, M. Holtzapple, and M. Ladisch (2005) Features of promising technologies for pretreatment of lignocellu losic biomass. Bioresour. Technol. 96: 673–686.
Torget, R., P. Walter, M. Himmel, and K. Grohmann (1991) Dilute-acid pretreatment of corn residues and short-rotation woody crops. Appl. Biochem. Biotechnol. 28–29: 75–86.
Sang, W. B., J. S. Kim, Y. K. Park, Y. S. Kim, and K. K. Oh (2008) The effect of sugar decomposed on the ethanol fermentation and decomposition reactions of sugars. Biotechnol. Bioprocess Eng. 13: 332–341.
Hsu, T. A. (1996) Pretreatment of biomass. pp. 179–195. In: C. E. Wyman (ed.). Handbook on Bioethanol: Production and Utilization. Taylor & Francis, Washington DC, USA.
Gould, J. M. (1985) Studies on the mechanism of alkaline peroxide delignification of agricultural residues. Biotechnol. Bioeng. 27: 225–231.
Martel, P. and J. M. Gould (1990) Cellulose stability and delignification after alkaline hydrogen-peroxide treatment of straw. J. Appl. Poly. Sci. 39: 707–714.
Yang, B., A. Boussaid, S. D. Mansfield, D. J. Gregg, and J. N. Saddler (2002) Fast and efficient alkaline peroxide treatment to enhance the enzymatic digestibility of steam-exploded softwood substrates. Biotechnol. Bioeng. 77: 678–684.
Kim, S. B., B. H. Um, and S. C. Park (2001) Effect of pretreatment of reagent and hydrogen peroxide on enzymatic hydrolysis of oak in percolation process. Appl. Biochem. Biotechnol. 91–93: 81–94.
Neely, W. C. (1984) Factors affecting the pretreatment of biomass with gaseous ozone. Biotechnol. Bioeng. 26: 59–65.
Sun, Y. and J. J. Cheng (2002) Hydrolysis of lignocellulosic materials for ethanol production: a review. Biore sour. Technol. 83: 1–11.
Yang, B. and C. E. Wyman (2004) Effect of xylan and lignin removal by batch and flow through pretreatment on the enzymatic digestibility of corn stover cellulose. Biotechnol. Bioeng. 86: 88–95.
Choi, G. W., H. W. Kang, Y. R. Kim, and B. W. Chung (2008) Ethanol production by Zymomonas mobilis CHZ2501 from industrial starch feedstocks. Biotechnol. Bioprocess Eng. 13: 765–771.
National Renewable Energy Laboratory, Standard Biomass Analytical Procedures. http://www.nrel.gov/biomass/analytical_procedures.html.
Agblevor, F. A., S. Batz, and J. Trumbo (2003) Composition and ethanol production potential of cotton gin residues. Appl. Biochem. Biotechnol. 105: 219–230.
Larsson, S., A. Quintana-Sainz, A. Reimann, N. O. Nilvebrant, and L. T. Jonsson (2000) Influence of lignocellulose-derived aromatic compounds on oxygenlimited growth and ethanolic fermentation by Saccharomyces cerevisiae. Appl. Biochem. Biotechnol. 84–86: 617–632.
Taherzadeh, M. J., C. Niklasson, and G. Lidén (1999) Conversion of dilute-acid hydrolyzates of spruce and birch to ethanol by fed-batch fermentation. Bioresour. Technol. 69: 59–66.
Dien, B. S., E. A. Ximenes, P. J. O’Bryan, M. Moniruzzaman, X.-L. Li, V. Balan, B. Dale, and M. A. Cotta (2008) Enzyme characterization for hydrolysis of AFEX and liquid hot-water pretreated distillers’ grains and their conversion to ethanol. Bioresour. Technol. 99: 5216–5525
Kim, T. H., F. Taylor, and K. B. Hicks (2008) Bioethanol production from barley hull using SAA (soaking in aqueous ammonia) pretreatment. Bioresour. Technol. 99: 5694–5702.
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Han, M., Moon, SK., Kim, Y. et al. Bioethanol production from ammonia percolated wheat straw. Biotechnol Bioproc E 14, 606–611 (2009). https://doi.org/10.1007/s12257-008-0320-0
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DOI: https://doi.org/10.1007/s12257-008-0320-0