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Direct purification of Burkholderia Pseudomallei lipase from fermentation broth using aqueous two-phase systems

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Abstract

An aqueous two-phase purification process was employed for the recovery of Burkholderia pseudomallei lipase from fermentation broth. The partition behavior of B. pseudomallei lipase was investigated with various parameters such as phase composition, tie-line length (TLL), volume ratio (VR), sample loading, system pH, and addition of neutral salts. Optimum conditions for the purification of lipase were obtained in polyethylene glycol (PEG) 6000-potassium phosphate system using TLL of 42.2% (w/w), with VR of 2.70, and 1% (w/w) NaCl addition at pH 7 for 20% (w/w) crude load. Based on this system, the purification factor of lipase was enhanced to 12.42 fold, with a high yield of 93%. Hence, the simplicity and effectiveness of aqueous two-phase systems (ATPS) in the purification of lipase were proven in this study.

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Authors and Affiliations

  1. Department of Process and Food Engineering, Universiti Putra Malaysia, 43400, UPM Serdang, Selangor, Malaysia

    Chien Wei Ooi, Siti Mazlina Mustapa Kamal & Tau Chuan Ling

  2. Department of Chemical and Environmental Engineering, Universiti Putra Malaysia, 43400, UPM Serdang, Selangor, Malaysia

    Beng Ti Tey

  3. Faculty of Engineering and Science, Universiti Tunku Abdul Rahman, 53300, Setapak, Kuala Lumpur, Malaysia

    Siew Ling Hii

  4. Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, UPM Serdang, Selangor, Malaysia

    Arbakariya Ariff

  5. Department of Chemical Engineering and Materials Science, College of Engineering, Yuan Ze University, Yuan Ze, Taiwan

    Ho Shing Wu, John Chi Wei Lan & Ruey Shin Juang

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  1. Chien Wei Ooi
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Ooi, C.W., Tey, B.T., Hii, S.L. et al. Direct purification of Burkholderia Pseudomallei lipase from fermentation broth using aqueous two-phase systems. Biotechnol Bioproc E 14, 811–818 (2009). https://doi.org/10.1007/s12257-008-0306-y

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