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Gene-expression analysis of acidic pH shock effects on two-component systems in Streptomyces coelicolor

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Abstract

In this study, transcriptional analyses by using reverse transcription-polymerase chain reaction (RT-PCR), DNA chip, and quantitative real time-PCR were performed to investigate effects of acidic pH shock on two-component systems in Streptomyces coelicolor A3(2). Two-component systems of cseC/cseB and vanS/vanR known to be closely linked with self-protection against cell wall hydrolysis caused by external stimuli were upregulated by the pH shock. The chiS/chiR, afsQ2/afsQ1, ecrA2/ecrA1, bldM, ramC/ramR, and ragK/ragR known to be positively associated with the initiation of secondary metabolism were also upregulated. The cutS/cutR known to be negatively related to the secondary metabolism was, however, slightly downregulated. Upregulation or downregulation by the acidic pH shock of these two-component regulator systems might have contributed in a concerted manner to the enhancement of secondary metabolite production, at least, in this particular case of actinorhodin production in S. coelicolor A3(2).

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Authors and Affiliations

  1. Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Daejeon, 305-701, Korea

    Yoon Jung Kim, An Na Moon, Jae Yang Song & Yong Keun Chang

  2. Department of Biological Engineering, Inha University, Incheon, 402-751, Korea

    Eung Soo Kim

  3. Department of Chemical Engineering, Gyeongsang National University, Jinju, 660-701, Korea

    Chang Joon Kim

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  1. Yoon Jung Kim
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Correspondence to Yong Keun Chang.

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Kim, Y.J., Moon, A.N., Song, J.Y. et al. Gene-expression analysis of acidic pH shock effects on two-component systems in Streptomyces coelicolor . Biotechnol Bioproc E 14, 584–590 (2009). https://doi.org/10.1007/s12257-008-0260-8

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  • DOI: https://doi.org/10.1007/s12257-008-0260-8

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