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Statistical optimization of culture media and conditions for production of mannan by Saccharomyces cerevisiae

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Abstract

In view of the increase in Saccharomyces cerevisiae mannan content, the culture medium and condition for S. cerevisiae were optimized in this study. The influence of culture medium ingredients such as carbon and nitrogen sources, inorganic ion, and enzyme activator on mannan production were evaluated using factional design. The mathematical model was established by the quadratic rotary combination design through response surface analysis. The optimized concentrations of culture medium were determined as follows: 4.98 g/100 mL, sucrose; 4.39 g/100 mL, soybean peptone; 3.10 g/100 mL, yeast extract; and 2.21 g/100 mL, glycerol. The optimized culture medium increased mannan production from 82.7 ± 3.4 mg/100 mL to 162.53 ± 3.47 mg/100 mL. The influence of original pH, inoculum size, temperature, and media volume on mannan production was evaluated and confirmed by orthogonale experimental design, with the order of effect as follows: media volume > temperature > initial pH > inoculation size. The optimized culture condition was pH, 5; inoculum size, 5 ml; temperature, 32°C; and media volume, 40 mL. The maximum mannan production increased to 258.5 ± 9.1 mg/100 mL at the optimum culture condition. It was evident that the mannan production was affected significantly by culture medium and condition optimization (p < 0.01).

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Authors and Affiliations

  1. Institute of Agro-food Science and Technology, The Key Laboratory of Agro-food Process and Quality Control, Ministry of Agriculture, Chinese Academy of Agriculture Sciences, Beijing, 100-193, China

    Hong-Zhi Liu, Qiang Wang, Yuan-Yuan Liu & Fang Fang

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  1. Hong-Zhi Liu
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  2. Qiang Wang
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  3. Yuan-Yuan Liu
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  4. Fang Fang
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Correspondence to Qiang Wang.

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Liu, HZ., Wang, Q., Liu, YY. et al. Statistical optimization of culture media and conditions for production of mannan by Saccharomyces cerevisiae . Biotechnol Bioproc E 14, 577–583 (2009). https://doi.org/10.1007/s12257-008-0248-4

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  • DOI: https://doi.org/10.1007/s12257-008-0248-4

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