Production of β-carotene by recombinant Escherichia coli with engineered whole mevalonate pathway in batch and fed-batch cultures

  • Jung Hun Kim
  • Seon-Won Kim
  • Do Quynh Anh Nguyen
  • He Li
  • Sung Bae Kim
  • Yang-Gon Seo
  • Jae-Kyung Yang
  • In-Young Chung
  • Dae Hwan Kim
  • Chang-Joon KimEmail author


Recombinant Escherichia coli engineered to contain the whole mevalonate pathway and foreign genes for β-carotene biosynthesis, was utilized for production of β-carotene in bioreactor cultures. Optimum culture conditions were established in batch and pH-stat fed-batch cultures to determine the optimal feeding strategy thereby improving production yield. The specific growth rate and volumetric productivity in batch cultures at 37°C were 1.7-fold and 2-fold higher, respectively, than those at 28°C. Glycerol was superior to glucose as a carbon source. Maximum β-carotene production (titer of 663 mg/L and overall volumetric productivity of 24.6 mg/L × h) resulted from the simultaneous addition of 500 g/L glycerol and 50 g/L yeast extract in pH-stat fed-batch culture.


recombinant Escherichia coli engineered whole mevalonate pathway β-carotene bioreactor culture fed-batch culture 


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Copyright information

© The Korean Society for Biotechnology and Bioengineering and Springer Berlin Heidelberg 2009

Authors and Affiliations

  • Jung Hun Kim
    • 1
  • Seon-Won Kim
    • 2
  • Do Quynh Anh Nguyen
    • 1
  • He Li
    • 1
  • Sung Bae Kim
    • 1
  • Yang-Gon Seo
    • 1
  • Jae-Kyung Yang
    • 3
  • In-Young Chung
    • 4
  • Dae Hwan Kim
    • 5
  • Chang-Joon Kim
    • 1
    Email author
  1. 1.Department of Chemical & Biological Engineering and ERIGyeongsang National UniversityJinjuKorea
  2. 2.Division of Applied Life Science (BK21), EB-NCRC and PMBBRCGyeongsang National UniversityJinjuKorea
  3. 3.Division of Environmental Forest Science and Institute of Agriculture & Life ScienceGyeongsang National UniversityJinjuKorea
  4. 4.Department of Electronic and Communications EngineeringKwangwoon UniversitySeoulKorea
  5. 5.School of Electrical EngineeringKookmin UniversitySeoulKorea

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