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Enhanced continuous production of lovastatin using pellets and siran supported growth of Aspergillus terreus in an airlift reactor

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Lovastatin, a hypocholesterolemic agent, is a secondary metabolite produced by filamentous microorganism Aspergillus terreus in submerged batch cultivation. Lovastatin production by pellets and immobilized siran cells was investigated in an airlift reactor. The process was carried out by submerged cultivation in continuous mode with the objective of increasing productivity using pellet and siran supported growth of A terreus. The continuous mode of fermentation improves the rate of lovastatin production. The effect of dilution rate and aeration rate were studied in continuous culture. The optimum dilution rate for pellet was 0.02 h−1 and for siran carrier was 0.025 h−1. Lovastatin productivity using immobilized siran carrier (0.0255 g/L/h) was found to be greater than pellets (0.022 g/L/h). The productivity by both modes of fermentation was found higher than that of batch process which suggests that continuous cultivation is a promising strategy for lovastatin production.

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  1. Greenspan, M. D. and J. B. Yudkovitz (1985) Mevinolinic acid biosynthesis by Aspergillus terreus and its relationship to fatty acid biosynthesis. J. Bacteriol. 162: 704–707.

    CAS  Google Scholar 

  2. Manzoni, M., M. Rollini, S. Bergomi, and V. Cavazzoni (1998) Production and purification of statins from Aspergillus terreus strains. Biotechnol. Techniques 12: 529–532.

    Article  CAS  Google Scholar 

  3. Srivastava, P. and S. Kundu (1998) A comparative evaluation of Cephalosporin C production using various immobilization modes. J. Gen. Appl. Microbiol. 44: 113–117.

    Article  CAS  Google Scholar 

  4. Kumar, M. S., S. V. Kumar, and A. K. Sadhukhan (2000) Repeated fed batch process for improving Lovastatin production. Proc. Biochem. 36: 363–368.

    Article  CAS  Google Scholar 

  5. Novak, K., S. Gerdin, and M. Berovic (1997) Increased lovastatin formation by Aspergillus terreus using repeated fed batch process. Biotech. Letter. 19: 947–948.

    Article  CAS  Google Scholar 

  6. Seiskari, P., Y. Y. Linko, and P. Linko (1985) Continuous production of gluconic acid by immobilized Gluconobacter oxydans cell bioreactor. Appl. Microbiol. Biotechnol. 21: 356–360.

    Article  CAS  Google Scholar 

  7. Samiee, M. S., N. Moazami, S. Haghighi, F. A. Mohseni, S. Mirdamadi, and M. R. Bakhtiari (2003) Screening of lovastatin production by filamentous fungi. Biomed. J. 7: 29–33.

    CAS  Google Scholar 

  8. Miller, G. L. (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31: 426–428.

    Article  CAS  Google Scholar 

  9. Dubois, M., K. A. Gilles, J. K. Hamilton, P. A. Rebers, and F. Smith (1956) Colorimetric method for determination of sugars and related substances. Anal. Chem. 28: 350–356.

    Article  CAS  Google Scholar 

  10. Kysilka, R. and V. Kren, (1993) Determination of Lovastatin (mevinolin) and mevinolinic acid in fermentation liquids. J. Chromatogr. 630: 415–417.

    Article  Google Scholar 

  11. Friedrich, J., M. Zuzek, M. Bencina, A. Cimermen, A. Strancar, and I. Radez (1995) High performance liquid chromatographic analysis of mevinolin as mevinolinic acid in fermentation broths. J. Chromatogr. 704: 363–367.

    Article  CAS  Google Scholar 

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Correspondence to Pradeep Srivastava.

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Gupta, K., Mishra, P.K. & Srivastava, P. Enhanced continuous production of lovastatin using pellets and siran supported growth of Aspergillus terreus in an airlift reactor. Biotechnol Bioproc E 14, 207–212 (2009).

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