Summary
For decades, polymerase chain reaction (PCR) has been playing a fundamental role in hematology. Not only in diagnostics, but also in follow-up and therapeutic decision-making, PCR impacts the treatment of patients with neoplastic diseases. In this educational, commonly used PCR methods are explained. In addition, the strengths and weaknesses of PCR in the clinical setting are illustrated.
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References
Mullis KB, Faloona FA. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods Enzymol. IEEE Trans Med Imaging. 1987;155:335:50.
Bench AJ. The role of molecular genetic analysis within the diagnostic haemato-oncology laboratory. Int J Lab Hematol. 2012;34(1):21–34.
Alikian M, Gale RP, Apperly JF, Foroni L. Molecular techniques for the personalized management of patients with chronic myeloid leukemia. Biomol Detect Quant. IEEE Trans Med Imaging. 2017;11:4:20.
Rawer D, Borkhardt A, Wilda M, Kropf S, Kreuder J. Influence of stochastics on quantitative PCR in the detection of minimal residual disease. Leukemia. 2003;17:2527–44.
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O. Zach declares that he has no competing interests.
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Zach, O. Educational no. 4: PCR-based methods. memo 12, 4–6 (2019). https://doi.org/10.1007/s12254-018-0460-7
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DOI: https://doi.org/10.1007/s12254-018-0460-7