Summary
Flow cytometry (FC) plays an important role in the diagnosis and classification of hematological disorders and can analyze a wide range of diagnostic samples. It is used in both diagnostic and follow-up testing, and is increasingly important in the detection of very small residual disease populations, i. e., minimal residual disease (MRD). Modern flow cytometers have the capability to analyze several thousands of cells per second and to identify different cell populations by evaluating the expression of specific antigens (markers) along with light scatter properties of the cells. FC can precisely differentiate between B‑ and T‑cell malignancies, between mature and precursor tumors, and among the latter, determine the myeloid or lymphatic lineage.
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References
Gorczyca W. Flow Cytometry in neoplastic hematology: morphologic-Immunophenotypic correlation. 3rd ed. Boca Raton: CRC Press; 2017.
Leach M, Drummond M, Doig A. Practical flow cytometry in haematology diagnosis. Hoboken: Wiley-Blackwell; 2013.
Brown M, Wittwer C. Flow cytometry: principles and clinical applications in hematology. Clin Chem. 2000;46:1221–9.
Givan AL. Principles of flow cytometry: an overview. Methods Cell Biol. 2001;63:19–50.
Leach M, Drummond M, Doig A, McKay P, Jackson B, Bain BJ. Practical flow Cytometry in Haematology : 100 worked examples. Hoboken: Wiley-Blackwell; 2015.
Gaipa G, Basso G, Biondi A, Campana D. Detection of minimal residual disease in pediatric acute lymphoblastic leukemia. Cytometry B Clin Cytom. 2013;84(6):359–69.
Wood BL. Principles of minimal residual disease detection for hematopoietic neoplasms by flow cytometry. Cytometry B Clin Cytom. 2016;90:47–53.
Theunissen P, Mejstrikova E, Sedek L, van der Slujjs-Gelling AJ, Gaipa G, Bartels M, et al. Standardized flow cytometry for highly sensitive MRD measurements in B‑cell acute lymphoblastic leukemia. Blood. 2017;129(3):347–57.
Keeney M, Hedley BD, Chin-Yee ICH. Flow cytometry—Recognizing unusual populations in leukemia and lymphoma diagnosis. Int J Lab Hematol. 2017;39(Suppl 1):86–92.
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K. Hefler-Frischmuth declares that she has no competing interests.
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Hefler-Frischmuth, K. Educational no. 2: flow cytometry. memo 11, 168–171 (2018). https://doi.org/10.1007/s12254-018-0418-9
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DOI: https://doi.org/10.1007/s12254-018-0418-9