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Association between MICA rs2596542 Polymorphism with the Risk of Hepatocellular Carcinoma in Chronic Hepatitis C Patients

  • Original Article
  • Published:
Pathology & Oncology Research

Abstract

In this study we investigated the impact of rs2596542A/G single nucleotide polymorphism (SNP) in the major histocompatibility complex class I chain-related sequence A (MICA) gene on HCV-induced hepatocellular carcinoma (HCC) susceptibility in a Brazilian population. In total, 252 HCV-infected patients (98 with HCV-induced HCC and 154 non-malignant HCV-induced liver cirrhosis) were enrolled and 98 healthy control subjects (negative anti-HCV). The MICA rs2596542 SNP genotypes were determined by real-time PCR assay. No differences in MICA genotype frequencies between HCV-induced cirrhosis patients and controls were observed. However, genotype frequencies of rs2596542A/G SNP were statistically different between HCV-induced HCC patients and controls (p = 0.048), and also between HCC and HCV-induced cirrhosis patients (p = 0.039). The highest frequency of the rs2596542AA genotype was observed in HCC patients (31.6%) when compared with HCV-induced cirrhosis patients (18.8%) and healthy controls (19.4%). Also, rs2596542AA genotype carriers have an increased risk for HCC when compared to HCV-induced cirrhosis status [odds ratio (OR) = 1.99; 95% confidence interval (CI) = 1.06–3.74, p = 0.020)] and healthy individuals (OR = 1.92, 95% CI = 1.00–3.70, p = 0.049). Taken together our study suggest that MICA rs2596542 SNP impacts HCV-induced HCC susceptibility suggesting that genetic variants in MICA are of clinical relevance to hepatocarcinogenesis by impacting host immune response in chronic HCV infection.

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Acknowledgments

The authors thank the patients of the Hospital de Clínicas de Porto Alegre for their collaboration on this study.

Funding

This work was supported by the Universidade Luterana do Brasil, Hospital de Clínicas de Porto Alegre, and by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES - Finance Code 001).

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Authors and Affiliations

  1. Human Molecular Genetics Laboratory, Universidade Luterana do Brasil (ULBRA), Canoas, RS, Brazil

    Camila Guerra Marangon, Jóice Teixeira de Bitencorte, Rafael Tomoya Michita, Vagner Ricardo Lunge & Daniel Simon

  2. Serviço de Gastroenterologia, Departamento de Medicina Interna, Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil

    Deivid Cruz dos Santos & Mário Reis Álvares-da-Silva

  3. PPG Biologia Celular e Molecular Aplicada à Saúde, Universidade Luterana do Brasil, Av. Farroupilha, 8001 – Prédio 22 – 5° andar, Canoas, RS, 92425-900, Brazil

    Daniel Simon

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  1. Camila Guerra Marangon
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Contributions

Conceived and designed the experiments: CGM MRAS DS. Performed the experiments: CGM JTB DCS. Analyzed the data: CGM MRAS RTM DS. Contributed reagents/analysis tools: VRL. Wrote the paper: CGM RTM DS.

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Marangon, C.G., de Bitencorte, J.T., Michita, R.T. et al. Association between MICA rs2596542 Polymorphism with the Risk of Hepatocellular Carcinoma in Chronic Hepatitis C Patients. Pathol. Oncol. Res. 26, 1519–1525 (2020). https://doi.org/10.1007/s12253-019-00738-6

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