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The Involvement of NF-κB/Klotho Signaling in Colorectal Cancer Cell Survival and Invasion

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Pathology & Oncology Research

Abstract

Lipopolysaccharide significantly increased invasion, cell proliferation, and phospho-NF-κB p65 and phospho-IGF-1R protein, but decreased klotho protein expression, cell apoptosis, and the percentage of sub G0/G1 cells in SW480 and HT29 colorectal cancer cells. In contrast, NF-κB inhibitor exhibited a counteract effect of lipopolysaccharide. Transfection of Toll-like receptor 4 shRNA significantly decreased phospho-NF-κB p65 and phospho-IGF-1R protein levels, invasion, but significantly increased klotho protein expression, cell apoptosis, and the percentage of sub G0/G1 in SW480 and HT29 cells. In conclusion, inflammation inhibits klotho gene expression in colorectal cancer cells through activation of Toll-like receptor 4 /NF-κB signal pathway.

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Acknowledgements

This study was supported by the Postdoctoral Science Foundation of China (2016 M602416), National Natural Science Foundation of China (81704089), Postdoctoral Science Fund Special Grant of China (2017 T100603), 2016 Postdoctoral Researcher Daily Funding of Hunan Province (49), the Department of Education Key Project of Hunan Province (15A024), and Changsha City’s Outstanding Innovation Youth Training Program (kq 1707012).

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  1. Biao Xie and Shaolin Nie contributed equally to this work.

Authors and Affiliations

  1. Department One of Anorectal Surgery, The Second Affiliated Hospital of Hunan University of Chinese Medicine, 139 Renmin Road, Changsha, Hunan, 410005, People’s Republic of China

    Biao Xie, Fan Hu, Mei Li & Yongheng He

  2. Department of Colorectal Surgery, Hunan Province Tumor Hospital, Changsha, 410013, Hunan, China

    Shaolin Nie

  3. Department of Gastrointestinal Surgery, The Third Xiangya Hospital, Central South University, Changsha, 410013, Hunan, China

    Gui Hu

  4. Department of Hepatobiliary Surgery, the Second Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China

    Li Xiong

  5. Hunan Slack King Laboratory Animal Co., Ltd., Changsha, 4100125, Hunan, China

    Tianshu Peng

  6. Department Four of Anorectal Surgery, The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan, 410005, People’s Republic of China

    Jing Nie

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  1. Biao Xie
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Correspondence to Yongheng He.

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Xie, B., Nie, S., Hu, G. et al. The Involvement of NF-κB/Klotho Signaling in Colorectal Cancer Cell Survival and Invasion. Pathol. Oncol. Res. 25, 1553–1565 (2019). https://doi.org/10.1007/s12253-018-0493-6

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