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Protein Expression Analysis in Uterine Cervical Cancer for Potential Targets in Treatment

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Pathology & Oncology Research

Abstract

Specific markers in lesions of the human uterine cervix cancer (UCC) are still needed for prognostic, diagnostic and/or therapeutic purposes. In this study we evaluated key molecules at protein level between normal epithelium, cervical intraepithelial neoplasia (CIN1–3) and invasive cancer of a group of molecules previously reported at mRNA level. For that purpose, human formalin-fixed paraffin embedded tissue microarrays (TMAs) were constructed containing 205 Mexican tissue core specimens. Immunohistochemistry and quantitative analysis of histological staining was performed against twenty-two distinct proteins for each core and the processing platform ImageJ. In the progression of the disease we found key statistical differences for the proteins SEL1, Notch3 and SOCS3. High expressions of SEL1L, Notch3 and SOCS3 have potential value to increase the prognostic of UCC in combination with markers such as p16INK4a. This study identified key drivers in cervical carcinogenesis that should be evaluated for the development of UCC therapies.

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Acknowledgments

We thank Dr. Cuauhtémoc Oros Ovalle (C.O.O) and Dr. Alfredo Loredo Ramírez (A.L.R.) for providing us the specimens and for helpful diagnostic confirmation respectively. We would also like to thank Dr. Peter B. Mandeville for conducting the statistical analyses.

Funding

This study was financially supported by the Mexican sectorial grant SSA/IMSS/ISSSTE-CONACYT under project number 2011–1-160607 (to L. Santos). Dr. Sugela Blancas, postdoctoral fellow and Rogelio Medina-Berlanga (M. Sc. scholarship no. 300170) received scholarships from Consejo Nacional de Ciencia y Tecnología (CONACYT, Mexico).

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Authors and Affiliations

  1. División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica, A.C. (IPICYT), San Luis Potosí, Mexico

    Sugela Blancas, Rogelio Medina-Berlanga & Leticia Santos

  2. Centro de Ciencias de la Salud, Universidad Autónoma de Aguascalientes, Aguascalientes, Mexico

    Sugela Blancas

  3. Facultad de Ingeniería en Biotecnología, Universidad Politécnica de Pénjamo, Pénjamo, Guanajuato, Mexico

    Liliana Ortíz-García

  4. Laboratorio de Patología Quirúrgica, Mariano Arista 743, Interior 208, San Luis Potosí, Mexico

    Alfredo Loredo-Ramírez

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  1. Sugela Blancas
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  2. Rogelio Medina-Berlanga
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  3. Liliana Ortíz-García
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  4. Alfredo Loredo-Ramírez
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  5. Leticia Santos
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Correspondence to Leticia Santos.

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Ethical Approval

The bioethical committee of this Institution (Comisión de Seguridad e Higiene y Medio Ambiente en el Trabajo, CSHyMA-IPICYT) approved the experimental design of this work under the letter no. 003.

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The manuscript does not contain any studies with human participants by any of the authors and archive cases remained anonymous.

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The authors declare that they have no conflict of interest.

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Blancas, S., Medina-Berlanga, R., Ortíz-García, L. et al. Protein Expression Analysis in Uterine Cervical Cancer for Potential Targets in Treatment. Pathol. Oncol. Res. 25, 493–501 (2019). https://doi.org/10.1007/s12253-018-0401-0

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  • DOI: https://doi.org/10.1007/s12253-018-0401-0

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